Biolegend, 343609, Brilliant Violet 421™ anti-human CD34 Antibody, 25tests

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glyco-phosphoprotein with an approximate molecular weight of 105-120 kD. It is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells. Based on different sensitivities, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
25tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: The 561 antibody recognizes a class III group epitope, which is resistant to sialidase/glycolyprotease and chymopapain treatment.
Application References(PubMed link indicates BioLegend citation): Croockewit AJ, et al. 1998. Scand. J. Immunol. 47:82. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
Product Citations: Scala S, et al. 2023. Nat Commun. 14:3068. PubMed Rosendahl Huber A, et al. 2022. STAR Protoc. 3:101361. PubMed Brandsma AM, et al. 2021. Blood Cancer Discov. 2:484. PubMed Yuzuriha A, et al. 2021. Methods Mol Biol. 2454:411. PubMed de Kanter JK, et al. 2021. Cell Stem Cell. 28:1726. PubMed Vasu S, et al. 2016. Blood. 127: 2879 - 2889. PubMed Brandsma AM, et al. 2021. Cancer Discov. . PubMed Tong J, et al. 2021. Cell Stem Cell. 28(3):502-513.e6. PubMed Lu YC, et al. 2018. Cell Rep. 25:2083. PubMed Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed Nafria M, et al. 2020. Cell Rep. 31:107691. PubMed Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed Evans WS, et al. 2020. Exp Physiol. 105:1408. PubMed Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed Rabold K, et al. 2022. Nat Commun. 13:6149. PubMed Schielke L, et al. 2022. Front Immunol. 13:916701. PubMed Nafria M, et al. 2020. STAR Protoc. 1:100130. PubMed Hasaart KAL, et al. 2022. iScience. 25:103736. PubMed Seo GY et al. 2018. Cell host & microbe. 24(2):249-260 . PubMed Ward D, et al. 2016. Haematologica. 101: 286 - 296. PubMed Serr I, et al. 2016. Nat Commun. 7:10991. PubMed Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed Crippa S, et al. 2019. J Clin Invest. 129:1566. PubMed Ainsworth RI, et al. 2022. Nat Commun. 13:6221. PubMed
RRID: AB_11147951 (BioLegend Cat. No. 343609) AB_2561358 (BioLegend Cat. No. 343610)
Structure: 105-120 kD single chain mucin-like glycoprotein
Distribution: Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
Function: Cell adhesion
Ligand/Receptor: L-selecin, E-selectin
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: CD Molecules
Gene ID: 947
UniProt: View information about CD34 on UniProt.org
Clone: 561
Regulatory Status: RUO
Other Names: Gp105-120, My10, Mucosialin
Isotype: Mouse IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.