Biolegend, 343802, Purified anti-human CD49c (integrin α3) Antibody, 100μg

CD49c is a 150 kD α integrin chain known as α3 integrin or VLA-3 α chain. It is a type I transmembrane glycoprotein which is proteolytically cleaved into two disulfide linked fragments of 125 kD and 30 kD. CD49c forms a heterodimer with integrin β1 (α3β1, CD49c/CD29, VLA-3) and is expressed by many types of adhesion cells, such as endothelial cells, epithelial cells, and dermal fibroblasts. Weak expression has been reported on leukocytes. VLA-3 plays a role in cell-cell and cell-matrix adhesion through binding Kalinin, collagen, laminin-1, laminin-5, entactin, and fibronectin.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human squamous cell carcinoma cell line SCC-9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIP, WB, IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications include: immunoprecipitation4, Western blotting5, and immunohistochemical staining of frozen tissue sections2.
Application References(PubMed link indicates BioLegend citation): Pattaramalai S, et al. 1996. Exp. Cell. Res. 222:281. Skubitz AP, et al. 1996. Am. J. Pathol. 148:1445. (IHC) Skubitz AP, et al. 1998. FEBS Lett. 426:386. Calzada MJ, et al. 2004. Circ. Res. 94:462. (IP) Zhang X, et al. 2003. Surgery. 133:429. (WB)
Product Citations: Cegarra C, et al. 2022. PLoS One. 17:e0274667. PubMed
RRID: AB_1731901 (BioLegend Cat. No. 343802)
Structure: 150 kD type I transmembrane glycoprotein, integrin family, associate with integrin β1(CD29)
Distribution: Endothelial cells, epithelial cells, dermal fibroblast
Function: adhesion
Ligand/Receptor: Kalinin, collagen, laminin-1, laminin-5 and entactin, fibronectin
Cell Type: Endothelial cells, Epithelial cells, Fibroblasts
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. John Wiley & Sons Inc.
Gene ID: 3675
UniProt: View information about CD49c on UniProt.org
Clone: ASC-1
Regulatory Status: RUO
Workshop: VI A002
Other Names: α3 integrin, integrin α3 chain, VLA-3 α chain, VLA3a, ITGA3
Isotype: Mouse IgG1, κ