Biolegend, 343903, PE anti-human CD166 Antibody, 25tests

CD166, also known as the CD6 ligand or the Activated Leukocyte Cell Adhesion Molecule (ALCAM), is a 100-105 kD transmembrane glycoprotein. It belongs to the Ig superfamily of proteins and expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently CD166 has also been used as a potential cancer stem cell marker. The antibody reacts with human activated leukocyte cell adhesion molecule (ALCAM).
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Cultured human thymic epithelial cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections and immunofluorescence.1
Application References(PubMed link indicates BioLegend citation): Pretzel D, et al. 2011. Arthritis Res. Ther. 13:R64. (IHC, IF, FC)
Product Citations: He Y, et al. 2023. Protein Cell. 14:202. PubMed Fielding CA et al. 2017. eLife. 6 pii: e22206. PubMed Radhakrishnan S, et al. 2019. Mol Med Rep. 20:813. PubMed Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed Weeks AM, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed Tu S, et al. 2020. Cellular Signalling. 73:109695. PubMed Guney-Esken G, et al. 2021. Stem Cell Res Ther. 12:287. PubMed Argüello RJ, et al. 2012. PLoS One. 7:e35966. PubMed Morton JJ, et al. 2018. Mol Carcinog. 57:1651. PubMed Liang C, et al. 2021. Cell Res. 31:187. PubMed Chulpanova DS, et al. 2020. Bioengineering (Basel). 7:00. PubMed Aldeghaither DS, et al. 2019. Cancer Immunol Res. 7:230. PubMed Wu CL, et al. 2021. Nat Commun. 12:362. PubMed Morton JJ, et al. 2020. Molecular Cancer Research. 19(2):346-357. PubMed Bi S, et al. 2020. Protein Cell. 0.79375. PubMed Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed Yang C et al. 2019. Int J Mol Med. 43(3):1395-1405 . PubMed Ayhan S, et al. 2021. Journal of Cell Science. 134(6):. PubMed Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed Xu L, et al. 2016. Cancer Res . 76(23):6901-6910. PubMed Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed Duclos GE, et al. 2019. Sci Adv. 5:eaaw3413. PubMed Fernandez HR, et al. 2018. Cell Death Differ. 25:1239. PubMed Guney-Esken G, et al. 2021. Methods Mol Biol. 2549:23. PubMed Fu L, et al. 2019. PLoS Biol. 17:e3000201. PubMed Thiel A, et al. 2015. Sci Rep. 5: 17685. PubMed
RRID: AB_2289303 (BioLegend Cat. No. 343903) AB_2289302 (BioLegend Cat. No. 343904)
Structure: A type I transmembrane glycoprotein belonging to the Ig superfamily of proteins.
Distribution: CD166 is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons.
Function: Play an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development.
Ligand/Receptor: CD6
Cell Type: Epithelial cells, Fibroblasts, Mesenchymal Stem Cells, Monocytes, Neurons, T cells
Biology Area: Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Aruffo A, et al. 1997. Immunol Today. 18(10):498 2. Patel DD, et al. 1995. J. Exp. Med. 181:2213 3. Bowen MA, et al. 1995. J. Exp. Med. 181:1563 4. Horst D, et al. 2009. Cancer Invest. 22:1
Gene ID: 214
UniProt: View information about CD166 on UniProt.org
Clone: 3A6
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: CD6 ligand, Activated Leukocyte Cell Adhesion Molecule (ALCAM)
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.