Biolegend, 344006, APC anti-human CD73 (Ecto-5'-nucleotidase) Antibody, 100tests

CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include:immunofluorescence3. Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.
Application References(PubMed link indicates BioLegend citation): Nakamura T, et al. 1993. J. Immunol. 151:6933. Liao J, et al. 2011. J Endod. 37:1217. PubMed Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF) Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
Product Citations: Liu T, et al. 2020. Biochem Biophys Res Commun. 532:385. PubMed Yüce M, et al. 2022. J Cell Biochem. 123:1966. PubMed Gilazieva Z, et al. 2022. Curr Issues Mol Biol. 44:5363. PubMed Tolcher AW, et al. 2023. J Immunother Cancer. 11: . PubMed Nushtaeva A, et al. 2023. Int J Mol Sci. 24: . PubMed Ragni E, et al. 2021. Cells. 10: . PubMed Guney-Esken G, et al. 2021. Stem Cell Res Ther. 12:287. PubMed Jeon SB, et al. 2022. Int J Stem Cells. Online ahead of print. PubMed Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed Morton JJ, et al. 2018. Mol Carcinog. 57:1651. PubMed Chulpanova DS, et al. 2020. Bioengineering (Basel). 7:00. PubMed Ragni E, et al. 2022. Biomolecules. 12:. PubMed Santos J, et al. 2013. J Transl Med. 11:18. PubMed Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed Li J, et al. 2021. Mol Med Rep. 1:23. PubMed Wang D, et al. 2020. J Cell Mol Med. 24:4212. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Xu H, et al. 2022. Cell Death Dis. 13:478. PubMed Bruveris FF, et al. 2020. Development. 147:00:00. PubMed Nazari-Shafti TZ, et al. 2020. Biomolecules. 10:00. PubMed Bordin A, et al. 2019. Stem Cells Int. 2019:1516746. PubMed Hopf A, et al. 2022. Bioengineering (Basel). 9:. PubMed Moreno-Fernandez M, et al. 2011. Blood. 117:5372. PubMed Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed Buschmann J, et al. 2013. Cytotherapy. 15:1098. PubMed Guney-Esken G, et al. 2021. Methods Mol Biol. 2549:23. PubMed Hofmann L, et al. 2022. Front Med (Lausanne). 9:904295. PubMed Xu L, et al. 2022. Int J Mol Sci. 23:. PubMed Siciliano C, et al. 2016. Stem Cell Res. 17: 342-351. PubMed Ran Y, et al. 2022. Stem Cell Res Ther. 13:507. PubMed Deng P, et al. 2020. Bone Res. 8:39. PubMed Safi R, et al. 2021. Photodermatol Photoimmunol Photomed. 37:202. PubMed Papait A, et al. 2022. Front Immunol. 13:960909. PubMed
RRID: AB_1877158 (BioLegend Cat. No. 344005) AB_1877157 (BioLegend Cat. No. 344006)
Structure: GPI-linked 5'-nucleotidase, 70 kD
Distribution: Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells
Function: Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type: B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication. 2. Airas L and Jalkanen S, et al. 1996. Blood 88:1755. 3. Gutensohn W, et al. 1995. Cell Immunol. 161:213. 4. Airas L, et al. 1995. J. Exp. Med. 182:1603.
Gene ID: 4907
UniProt: View information about CD73 on UniProt.org
Clone: AD2
Regulatory Status: RUO
Workshop: V B-CD73.3
Other Names: Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Isotype: Mouse IgG1, κ