Biolegend, 344014, PerCP/Cyanine5.5 anti-human CD73 (Ecto-5'-nucleotidase) Antibody, 100tests

CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include:immunofluorescence3. Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.
Application References(PubMed link indicates BioLegend citation): Nakamura T, et al. 1993. J. Immunol. 151:6933. Liao J, et al. 2011. J Endod. 37:1217. PubMed Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF) Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
Product Citations: Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed Kameishi S, et al. 2023. Sci Rep. 13:4421. PubMed Gomzikova MO, et al. 2019. Cells. 0.440972222. PubMed Zheng W, et al. 2021. Ann Transl Med. 9:1148. PubMed Yang C et al. 2019. Int J Mol Med. 43(3):1395-1405 . PubMed OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
RRID: AB_2561756 (BioLegend Cat. No. 344013) AB_2561757 (BioLegend Cat. No. 344014)
Structure: GPI-linked 5'-nucleotidase, 70 kD
Distribution: Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells
Function: Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type: B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication. 2. Airas L and Jalkanen S, et al. 1996. Blood 88:1755. 3. Gutensohn W, et al. 1995. Cell Immunol. 161:213. 4. Airas L, et al. 1995. J. Exp. Med. 182:1603.
Gene ID: 4907
UniProt: View information about CD73 on UniProt.org
Clone: AD2
Regulatory Status: RUO
Workshop: V B-CD73.3
Other Names: Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.