Biolegend, 345001, Purified anti-human CD366 (Tim-3) Antibody, 25μg

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Tim-3 fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.
Application References(PubMed link indicates BioLegend citation): Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim) Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block) Klibi J, et al 2009. Blood 113:1957. (FC, Block)
Product Citations: Alpert A, et al. 2022. Cell Syst. 13:71. PubMed Jaiswal A, et al. 2022. Cancer Cell. 40:524. PubMed Wang H, et al. 2022. Front Immunol. 13:852436. PubMed Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed Eastman AJ, et al. 2021. Am J Reprod Immunol. 85:e13352. PubMed Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed Gee MH, et al. 2018. Cell. 172:549. PubMed Tao J, et al. 2020. J Cell Mol Med. 24:1046. PubMed Zhou H, et al. 2017. Leukemia. 31:2065. PubMed Ascierto ML, et al. 2017. Clin Cancer Res. 23:3168. PubMed
RRID: AB_2116575 (BioLegend Cat. No. 345001) AB_2116574 (BioLegend Cat. No. 345002)
Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
Distribution: Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells
Function: Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance
Ligand/Receptor: Galectin-9
Cell Type: Dendritic cells, Monocytes, T cells, Th1, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699. 2. Zhu C, et al. 2005. Nat. Immunol. 6:1245. 3. Wang F, et al. 2009. Immunobiology 214:342.
Gene ID: 84868
UniProt: View information about CD366 on UniProt.org
Clone: F38-2E2
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype: Mouse IgG1, κ