Biolegend, 345014, PE/Cyanine7 anti-human CD366 (Tim-3) Antibody, 100tests

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Tim-3 fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.
Application References(PubMed link indicates BioLegend citation): Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim) Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block) Klibi J, et al 2009. Blood 113:1957. (FC, Block)
Product Citations: Li N, et al. 2023. Nat Commun. 14:1986. PubMed Qin L, et al. 2022. Sci Adv. 8:eabn3774. PubMed Lu T, et al. 2023. Cancer Immunol Immunother. :. PubMed Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed Babl N, et al. 2023. Front Oncol. 13:1107484. PubMed Zhou R, et al. 2020. Immunity. S1074-7613(20)30333-2.. PubMed Zhao Y, et al. 2021. Front Immunol. 12:665442. PubMed AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed Gao Y, et al. 2021. Oncogenesis. 10:62. PubMed Zhan Y, et al. 2021. JCI Insight. 6:. PubMed Fisher J, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.03.002. PubMed Jespersen H, et al. 2017. Nat Commun. 10.1038/s41467-017-00786-z. PubMed Hebbar N, et al. 2022. Nat Commun. 13:587. PubMed Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed Jin S, et al. 2020. Cell Res. 30:950. PubMed Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed Harper J, et al. 2021. Nat Commun. 12:2866. PubMed Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed Riberdy JM, et al. 2020. Mol Ther Methods Clin Dev. 1.146527778. PubMed
RRID: AB_2561719 (BioLegend Cat. No. 345013) AB_2561720 (BioLegend Cat. No. 345014)
Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
Distribution: Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells
Function: Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance
Ligand/Receptor: Galectin-9
Cell Type: Dendritic cells, Monocytes, T cells, Th1, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699. 2. Zhu C, et al. 2005. Nat. Immunol. 6:1245. 3. Wang F, et al. 2009. Immunobiology 214:342.
Gene ID: 84868
UniProt: View information about CD366 on UniProt.org
Clone: F38-2E2
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype: Mouse IgG1, κ