Biolegend, 345201, Purified anti-human integrin β5 Antibody, 25μg

Integrins are a family of transmembrane proteins. A total 17 integrin α chains and 8 β chains have been identified. The association of integrin α and β chains forms 25 integrin complexes which serve as extracellular matrix receptors and play important roles in cell adhesion and development. Integrin β5 is a 95 kD glycoprotein heterodimer with the αV subunit and is found on many types of tissue cells, such as epithelial cells, endothelial cells, keratinocytes, and osteoblastic cells. The αV/β5 integrin complex binds to vitronectin.
25μg
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Steinberg F, et al. 2012. J Cell Biol. 197:219. PubMed Fedr R, et al. 2023. Sci Rep. 13:4889. PubMed Zhang G, et al. 2019. FASEB J. 33:2823. PubMed
RRID: AB_2234046 (BioLegend Cat. No. 345202)
Structure: Integrin family, Ig superfamily, associates with αV subunit
Distribution: Epithelial cells, endothelial cells, keratinocytes, osteoblastic cells
Function: Cell adhesion
Ligand/Receptor: vitronectin
Cell Type: Endothelial cells, Epithelial cells, Osteoclasts
Biology Area: Immunology
Molecular Family: Adhesion Molecules
Antigen References: 1. Kim JP. et al. 1994. J. Bio. Chem. 269:26926 2. Cheng SL. et al. 2000. J. Cell Biochem. 77:265 3. Aplin JD. et al. 1996. Mol. Hum. Reprod. 2:527
Gene ID: 3693
UniProt: View information about Integrin beta 5 on UniProt.org
Clone: AST-3T
Regulatory Status: RUO
Other Names: integrin b5, integrin beta 5, ITGB5
Isotype: Mouse IgG2a, κ