Biolegend, 346010, Brilliant Violet 605™ anti-human CD169 (Sialoadhesin, Siglec-1) Antibody, 100tests

CD169, also known as Siglec-1 and Sialoadhesin (Sn), is a 210 kD type I single membrane-spanning glycoprotein. It is the largest member of the Siglec family, consisting of 1709 amino acids and belonging to the immunoglobulin superfamily. CD169 is expressed by macrophages and dendritic cells. By its affinity to α2,3-linked sialic acid, it is involved in macrophage binding to different cell types such as granulocytes, monocytes, NK, B and T cells. Several CD169 counter receptors, such as CD227 on human breast cancer cells, CD43 on T cells and CD206 on macrophages, have been reported.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Rhinovirus (HRV14) infected, monocyte derived-DCs
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting and inhibition of erythrocyte-rosetting with cells expressing CD169.
Application References(PubMed link indicates BioLegend citation): Kirchberger S, et al. 2005. J. Immunol. 175:1145. Schrauf C, et al. 2009. J. Immunol. 183:4440.
Product Citations: Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed Lu Q, et al. 2021. Immunity. 54(6):1304-1319.e9. PubMed
RRID: AB_2721538 (BioLegend Cat. No. 346009) AB_2721539 (BioLegend Cat. No. 346010)
Structure: Type I single membrane-spanning lectin, 1709 amino acids, 210 kD, belongs to the immunoglobulin superfamily
Distribution: Macrophages, dendritic cells
Function: Adhesion molecule
Interaction: α-2,3-linked sialic acid
Ligand/Receptor: CD227, CD43 and CD206
Bioactivity: Mediates sialic-acid dependent binding to granulocytes, monocytes, NK, B and T cells.
Cell Type: B cells, Dendritic cells, Macrophages
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules, Siglec Molecules
Antigen References: 1. Xiong YS, et al. 2009. Clin. Biochem. 42:1057. 2. Varki A, et al. 2009. Glycoconj J. 26:231. 3. Rempel H, et al. 2008. PLoS One. 3:e1967. 4. Crocker PR, et al. 2001. Trends Immunol. 22:337. 5. Hartnell A, et al. 2001. Blood 97:288.
Gene ID: 6614
UniProt: View information about CD169 on UniProt.org
Clone: 7-239
Regulatory Status: RUO
Other Names: Sialic acid binding Ig-like lectin 1 (Siglec-1)
Isotype: Mouse IgG1, κ