Biolegend, 346706, PE anti-human NKp80 Antibody, 100tests

NKp80, also known as killer cell lectin-like receptor subfamily F, member 1 (KLRF1), is a type II transmembrane C-type lectin-like receptor. NKp80 is expressed on the cell surface of nearly all NK cells as a homodimer with approximately 80 kD molecular mass. It is also found on a subset of effector memory CD8 T cells and γ/δ T cells. NKp80 induces NK-cell mediated cytotoxicity and cytokine production through interaction with its ligand, AICL (activation-induced C-type lectin), which is selectively expressed on myeloid cells.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant ectodomain of human NKp80
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Welte S, et al. 2006. Nat. Immunol. 7:1334. Kuttruff S, et al. 2009. Blood 113:358.
Product Citations: Loyon R, et al. 2016. J Immunol. 197: 85 - 96. PubMed Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed Manser AR, et al. 2019. J Immunol. 203:2301. PubMed Chen M, et al. 2021. Cancers (Basel). 13:. PubMed Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
RRID: AB_1967147 (BioLegend Cat. No. 346706)
Structure: Type II transmembrane c-type lectin-like receptor, homodimer, 80 kD
Distribution: NK cells, subset of CD8 T and γ/δ T cells
Ligand/Receptor: AICL (activation-induced C-type letcin)
Bioactivity: induce NK cell-mediated cytolysis and cytokine release
Cell Type: NK cells, T cells
Biology Area: Costimulatory Molecules, Immunology, Innate Immunity
Antigen References: 1. Kuttruff S, et al. 2009. Blood 113:358 2. Vitale M, et al. 2001. Eur. J. Immunol. 31:233
Gene ID: 51348
UniProt: View information about NKp80 on UniProt.org
Clone: 5D12
Regulatory Status: RUO
Other Names: KLRF1, killer cell lectin-like receptor subfamily F, member 1
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.