Biolegend, 347105, APC anti-human Siglec-8 Antibody, 25tests

Siglec-8 is a lectin specific for 6'-sulfo-sLe x and a member of the Ig-superfamily. It is expressed almost exclusively in eosinophils; however, basophils and mast cells can express it to a lower degree. Siglec-8 is a 54 kD transmembranal protein; the extracellular domain has one V-set Ig-like domain and two C2-set domains. The cytoplasmic domain has two immunoreceptor tyrosine-based inhibitor motifs (ITIM) that recruit SH2-family phosphatases after tyrosine phosphorylation. There are reports that siglec-8 inhibits the release of histamine and prostaglandin D2 mediated by the IgEFcR. This molelcule is also involved in the induction of apoptosis.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Siglec-8 fused to human IgG Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Floyd H, et al. 2000. J. Biol. Chem. 275:861. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations: Toepfner N et al. 2018. eLife. 7 pii: e29213. PubMed Wu X, et al. 2023. iScience. 26:106559. PubMed Patra V, et al. 2019. Br J Dermatol. N/A. PubMed Gravastrand CS, et al. 2019. J Immunol. 203(4). PubMed Simon SCS, et al. 2020. Oncoimmunology. 9:1727116. PubMed Kim JE, et al. 2021. Front Immunol. 11:593748. PubMed Morshed N, et al. 2020. Mol Syst Biol. 16:e9819. PubMed Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed Ehrens A, et al. 2021. Cell Reports. 34(2):108621. PubMed Plum T, et al. 2020. Immunity. 52(2):404-416. PubMed
RRID: AB_2561401 (BioLegend Cat. No. 347105) AB_2561402 (BioLegend Cat. No. 347106)
Structure: A lectin of 54 kD, belonging to the Ig-superfamily; has one V-set Ig-like domain and two C2-set domains; has a single transmembrane domain, and two immunoreceptor tyrosine-based inhibitor motifs (ITIM) in the cytoplasmic domain
Distribution: Eosinophils, basophils, mast cells
Function: Cell adhesion, signal transduction
Interaction: Phosphatases containing a SH2 domain
Bioactivity: Inhibition of IgE receptor-triggered histamine and prostaglandin D2 release, apoptosis
Cell Type: Basophils, Eosinophils, Mast cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules, Siglec Molecules
Antigen References: 1. Bochner BS, et al. 2009. Clin. Exp. Allergy. 39:317. 2. Hudson SA, et al. 2009. J. Pharmacol. Exp. Ther. 330:608. 3. Nutku E, et al. 2005. Biochem. Biophys. Res. Commun. 336:918.
Gene ID: 27181
UniProt: View information about Siglec-8 on UniProt.org
Clone: 7C9
Regulatory Status: RUO
Other Names: Sialic acid-binding Ig-like lectin 8 (Siglec-8), Siglec8L, Sialoadhesin family member 2 (SAF2)
Isotype: Mouse IgG1, κ