Biolegend, 347128, KIRAVIA Blue 520™ anti-human Siglec-8 Antibody, 100tests

Siglec-8 is a lectin specific for 6'-sulfo-sLe x and a member of the Ig-superfamily. It is expressed almost exclusively in eosinophils; however, basophils and mast cells can express it to a lower degree. Siglec-8 is a 54 kD transmembranal protein; the extracellular domain has one V-set Ig-like domain and two C2-set domains. The cytoplasmic domain has two immunoreceptor tyrosine-based inhibitor motifs (ITIM) that recruit SH2-family phosphatases after tyrosine phosphorylation. There are reports that siglec-8 inhibits the release of histamine and prostaglandin D2 mediated by the IgEFcR. This molelcule is also involved in the induction of apoptosis.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Siglec-8 fused to human IgG Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm. KIRAVIA Blue™ 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.Sony and the Sony logo are registered trademarks of Sony Corporation.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Floyd H, et al. 2000. J. Biol. Chem. 275:861. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
RRID: AB_3068133 (BioLegend Cat. No. 347127) AB_3068133 (BioLegend Cat. No. 347128)
Structure: A lectin of 54 kD, belonging to the Ig-superfamily; has one V-set Ig-like domain and two C2-set domains; has a single transmembrane domain, and two immunoreceptor tyrosine-based inhibitor motifs (ITIM) in the cytoplasmic domain
Distribution: Eosinophils, basophils, mast cells
Function: Cell adhesion, signal transduction
Interaction: Phosphatases containing a SH2 domain
Bioactivity: Inhibition of IgE receptor-triggered histamine and prostaglandin D2 release, apoptosis
Cell Type: Basophils, Eosinophils, Mast cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules, Siglec Molecules
Antigen References: 1. Bochner BS, et al. 2009. Clin. Exp. Allergy. 39:317. 2. Hudson SA, et al. 2009. J. Pharmacol. Exp. Ther. 330:608. 3. Nutku E, et al. 2005. Biochem. Biophys. Res. Commun. 336:918.
Gene ID: 27181
UniProt: View information about Siglec-8 on UniProt.org
Clone: 7C9
Regulatory Status: RUO
Other Names: Sialic acid-binding Ig-like lectin 8 (Siglec-8), Siglec8L, Sialoadhesin family member 2 (SAF2)
Isotype: Mouse IgG1, κ