Biolegend, 348004, PE anti-human Granulysin Antibody, 100tests

Granulysin is a member of the saposin-like protein family of lipid binding proteins (SAPLIP). It is expressed by NK cells and cytolytic T lymphocytes (CTL) as 9 kD and 15 kD forms. The 9 kD form is derived from the 15 kD form by cleavage at both amino- and carboxy-termini. Granulysin has broad cytolytic activities against a variety of tumor cells and microbes, including Gram-positive and -negative bacteria, fungi and parasites. It also functions as a chemoattractant for inflammatory cells and activates the cells expression of cytokines and chemokines.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant 9 kD granulysin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone DH2 recognizes both the 9 kD and 15 kD isoforms of Granulysin.1ELISA Capture: To measure human granulysin, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated DH10 antibody (Cat. No. 526103) as the detection antibody. Recombinant Human Granulysin (ELISA Std.) (Cat. No. 552109) can be used as the protein standard.Flow Cytometry: The fluorochrome-labeled DH2 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify granulysin-producing cells within mixed cell populations.Note: For testing human granulysin in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Granulysin ELISA Kit with Pre-coated Plates (Cat. No. 438007 & 438008) are specially developed and recommended.
Application References(PubMed link indicates BioLegend citation): Hanson DA, et al. 1999. Mol. Immunol. 36:413. Spada FM, et al. 2000. J. Exp. Med. 191:937.
Product Citations: Sleiman M, et al. 2014. J Immunol. 2602:192. PubMed Abd Hamid M, et al. 2024. Nat Immunol. 834:25. PubMed Kalb M, et al. 2012. J Immunol. 188:1583. PubMed Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed Li Z, et al. 2020. NPJ Precis Oncol. 4:28. PubMed Davis Z, et al. 2016. PLoS Pathog. 12: 1005421. PubMed
RRID: AB_10559756 (BioLegend Cat. No. 348003) AB_2263307 (BioLegend Cat. No. 348004)
Structure: Saposin-like protein family of lipid binding protein, 9 kD and 15 kD
Distribution: NK cells, cytolytic T cells
Function: Cytolytic against bacteria, fungi, parasite and tumor cells; chemoattract inflammatory cells
Cell Type: NK cells, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Krensky AM. 2000. Biochem. Pharmacol. 59:317. 2. Deng A, et al. 2005. J. Immunol. 174:5243. 3. Kaspar AA, et al. 2001. J. Immunol. 167:350. 4. Krensky AM and Clayberger C. 2009. Tissue Antigens 73:193.
Gene ID: 10578
UniProt: View information about Granulysin on UniProt.org
Clone: DH2
Regulatory Status: RUO
Other Names: GNLY, 519, LAG2, NKG5, LAG-2, D2S69E, TLA519
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.