Biolegend, 348103, PE anti-human TCR Vβ8 Antibody, 25tests

Vβ8 T cell receptor (TCR Vβ8) is a variant of the TCR β chain which is heterodimerized with TCR α chain and expressed on a subset of T cells. In association with CD3 complex, TCR α/β functions recognition of antigen and initiating T cell mediated immune responses. Human Vβ8 has been described as the target of the streptococcal mitogenic exotoxin z (SMEZ) and SMEZ-2.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Lavoie PM, et al. 2005. Int. Immunol. 17:931 Okas M, et al. 2010. J. Immunother. 33:96
Product Citations: Ge Y, et al. 2019. J Immunol. 203:2827. PubMed Zhong S, et al. 2013. Proc Natl Acad Sci U S A. 110:6973. PubMed Malecek K, et al. 2013. J Immunol Methods. 392:1. PubMed Li F, et al. 2022. Nat Commun. 13:4334. PubMed
RRID: AB_10549976 (BioLegend Cat. No. 348104)
Structure: Immunoglobulin superfamily, associate with TCR Vα chain and CD3 complex
Distribution: Subset of T cells
Function: Antigen recognition
Ligand/Receptor: MHC-Peptide complex
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Proft T, et al. 1999. J. Exp. Med. 189:89. 2. Kelsen J, et al. 2004. Scand. J. Gastroenterol. 39:238.
Gene ID: 6957
UniProt: View information about TCR Vbeta8 on UniProt.org
Clone: JR2 (JR.2)
Regulatory Status: RUO
Other Names: Vbeta8
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.