Biolegend, 348501, Purified anti-human CD144 (VE-Cadherin) Antibody, 25μg

CD144, also known as VE-cadherin and cadherin-5, is a 140 kD glycoprotein which is composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region. It is a calcium-dependent transmembrane cell-cell adhesion molecule localized at the intercellular boundaries of endothelial cells, hematopoietic stem cells, and perineurial cells. It functions as a classic cadherin by mediating homophilic adhesion and functions as a plasma membrane attachment site for the cytoskeleton. CD144 is thought to play a role in vascular development, permeability, and remodeling.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICC - Verified WB, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 5.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.
Application Notes: Clone BV9 has been shown to block VE-cadherin, causing a redistribution of VE-cadherin away from intracellular junctions.6 This clone binds to EC3-EC4 region in the extracellular domain of human VE-cadherin.7 Additional reported applications (for the relevant formats) include: Western Blotting1,2, immunofluorescence microscopy1,3, immunoprecipitation1,4, blocking angiogenesis in vitro4,5, inhibiting VE-cadherin reorganization4, and inducing endothelial cell apoptosis4.
Application References(PubMed link indicates BioLegend citation): Almagro S, et al. 2010. Mol. Cell Biol. 30:1703. (WB, IF, IP) Zhang F, et al. 2004. J. Biol. Chem. 279:11760. (WB) Iurlaro M, et al. 2004. Am. J. Pathol. 165:181. (IF) Corada M, et al. 2001. Blood 97:1679. (IP, Block) Kooistra M, et al. 2005. FEBS 579:4966. (Block) Corada M, et al. 2001. Blood 97:1679. (Block) Bouillet L, et al. 2013. Laboratory Investigation 93:1194-11202.
Product Citations: Ghoroghi S, et al. 2021. Elife. 10:. PubMed Barzilai S, et al. 2017. Cell Rep. 18(3):685-699. PubMed Piao W, et al. 2020. Cell Reports. 30(4):1052-1062.e5.. PubMed Bashth OS, et al. 2020. Biomater Sci. 4.128472222. PubMed Boulanger MD, et al. 2022. PLoS One. 17:e0269316. PubMed Molina SG, et al. 2020. Stem Cell Res. 49:102015. PubMed Kondo N, et al. 2020. Cancers (Basel). 12:00. PubMed Liu X, et al. 2019. J Am Heart Assoc. 8:e013323. PubMed
RRID: AB_10574464 (BioLegend Cat. No. 348501) AB_10588267 (BioLegend Cat. No. 348502)
Structure: Member of the cadherin family; calcium-dependent transmembrane cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region
Distribution: Endothelial cells, hematopoietic stem cells, perineurial cells
Function: Mediates calcium-dependent homophilic cell adhesion, plays fundamental roles in microvascular permeability and in the morphogenic and proliferative events associated with angiogenesis
Ligand/Receptor: VE-cadherin
Cell Type: Endothelial cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
Antigen References: 1. Taddei A, et al. 2008. Nat. Cell Biol. 10:923. 2. Gavard J, et al. 2006. Nat. Cell Biol. 8:1223. 3. Kim I, et al. 2005. Blood 106:903. 4. Suzuki S, et al. 1991. Cell Regul. 2:261.
Gene ID: 1003
UniProt: View information about CD144 on UniProt.org
Clone: BV9
Regulatory Status: RUO
Other Names: VE-Cadherin, cadherin-5, CDH5
Isotype: Mouse IgG2a, κ