Biolegend, 348509, PerCP/Cyanine5.5 anti-human CD144 (VE-Cadherin) Antibody, 25tests

CD144, also known as VE-cadherin and cadherin-5, is a 140 kD glycoprotein which is composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region. It is a calcium-dependent transmembrane cell-cell adhesion molecule localized at the intercellular boundaries of endothelial cells, hematopoietic stem cells, and perineurial cells. It functions as a classic cadherin by mediating homophilic adhesion and functions as a plasma membrane attachment site for the cytoskeleton. CD144 is thought to play a role in vascular development, permeability, and remodeling.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone BV9 has been shown to block VE-cadherin, causing a redistribution of VE-cadherin away from intracellular junctions.6 This clone binds to EC3-EC4 region in the extracellular domain of human VE-cadherin.7 Additional reported applications (for the relevant formats) include: Western Blotting1,2, immunofluorescence microscopy1,3, immunoprecipitation1,4, blocking angiogenesis in vitro4,5, inhibiting VE-cadherin reorganization4, and inducing endothelial cell apoptosis4.
Application References(PubMed link indicates BioLegend citation): Almagro S, et al. 2010. Mol. Cell Biol. 30:1703. (WB, IF, IP) Zhang F, et al. 2004. J. Biol. Chem. 279:11760. (WB) Iurlaro M, et al. 2004. Am. J. Pathol. 165:181. (IF) Corada M, et al. 2001. Blood 97:1679. (IP, Block) Kooistra M, et al. 2005. FEBS 579:4966. (Block) Corada M, et al. 2001. Blood 97:1679. (Block) Bouillet L, et al. 2013. Laboratory Investigation 93:1194-11202.
Product Citations: Sierra-Parraga JM, et al. 2020. Stem Cell Res Ther. 0.702777778. PubMed
RRID: AB_2562927 (BioLegend Cat. No. 348509) AB_2562928 (BioLegend Cat. No. 348510)
Structure: Member of the cadherin family; calcium-dependent transmembrane cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region
Distribution: Endothelial cells, hematopoietic stem cells, perineurial cells
Function: Mediates calcium-dependent homophilic cell adhesion, plays fundamental roles in microvascular permeability and in the morphogenic and proliferative events associated with angiogenesis
Ligand/Receptor: VE-cadherin
Cell Type: Endothelial cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
Antigen References: 1. Taddei A, et al. 2008. Nat. Cell Biol. 10:923. 2. Gavard J, et al. 2006. Nat. Cell Biol. 8:1223. 3. Kim I, et al. 2005. Blood 106:903. 4. Suzuki S, et al. 1991. Cell Regul. 2:261.
Gene ID: 1003
UniProt: View information about CD144 on UniProt.org
Clone: BV9
Regulatory Status: RUO
Other Names: VE-Cadherin, cadherin-5, CDH5
Isotype: Mouse IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.