Biolegend, 348612, PerCP/Cyanine5.5 anti-human CD243 (MDR-1) Antibody, 100tests

CD243 (MDR-1) belongs to the ATP binding cassette (ABC) transporter family. With an approximate molecular mass of 170 kD, it consists of two homologous halves. Each half contains two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs). The TMDs span the membrane six times, forming a chamber with a 12 transmembrane α-helix structure. NBDs drive the transport process through ATP coupling and hydrolysis and are located at the cytoplasmic face of the membrane. CD243 transports various molecules across cellular membranes and is involved in multidrug resistance. MDR-1 is expressed on hematopoietic stem cells, T cells, B cells, and NK cells as well as on many multidrug resistant neoplastic cells. CD243 interacts with Caveolin, RING finger protein 1B, AAP1, p53, Orphan nuclear receptor PX, and cytochrome P450.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NIH 3T3 cells transfected with human MDR-1 cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: blocking the efflux of fluorescent dyes1,2, immunoprecipitation1, and immunohistochemical staining of tissue sections of squamous cell carcinoma3.
Application References(PubMed link indicates BioLegend citation): Mechetner EB and Roninson IB. 1992. Proc. Natl. Acad. Sci. USA 13:5824. (Block, IP) Chaudhary PM, et al. 1992. Blood 80:2735. (Block) Kelley DJ, et al. 1993. Arch. Otolaryngol. Head Neck Surg. 119:411. (IHC-P) Goda K, et al. 2007. J. Pharmacol. Exp. Ther. 320:81. (Block)
RRID: AB_2562766 (BioLegend Cat. No. 348611)
Structure: Member of ABC transporters family, MW ~170 kD. Consists of two homologous halves, each comprising two hydrophobic transmembrane domains (TMDs) and two hydrophilic nucleotide binding domains (NBDs).
Distribution: Hematopoietic stem cells, T and B cells, natural killer (NK) cells, and many multidrug resistant neoplastic cells.
Function: Transport various molecules across cellular membranes. Involved in multidrug resistance.
Cell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Dulucq S, et al. 2008. Blood 112:2024. 2. Gottesman MM, et al. 2009. Nat. Biotechnol. 27:546. 3. Andreadis C, et al. 2007. Blood 109:3409.
Gene ID: 5243
UniProt: View information about CD243 on UniProt.org
Clone: UIC2
Regulatory Status: RUO
Other Names: Multidrug resistance protein 1, ATP-binding cassette sub-family B member 1, P-glycoprotein 1
Isotype: Mouse IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.