Biolegend, 348701, FITC anti-human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56), 50tests

This anti-Human Lineage Cocktail is optimized for the detection of human lymphocytes, monocytes, eosinophils, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells (DCs), including DCs that express CD16. This cocktail is composed of CD3, CD14, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD19 and CD20 are on B cells; CD56 is located on NK cells.
50tests
Verified Reactivity: Human
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Wu X, et al. 2023. iScience. 26:106559. PubMed Ait Djebbara S, et al. 2023. Front Immunol. 14:1180900. PubMed Rosendahl Huber A, et al. 2022. STAR Protoc. 3:101361. PubMed Brandsma AM, et al. 2021. Blood Cancer Discov. 2:484. PubMed de Kanter JK, et al. 2021. Cell Stem Cell. 28:1726. PubMed Qian P et al. 2018. Cell stem cell. 22(5):740-754 . PubMed Brandsma AM, et al. 2021. Cancer Discov. . PubMed Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed Awad MM, et al. 2021. Front Oncol. 11:696512. PubMed Webb K et al. 2018. Frontiers in immunology. 2.574305556 . PubMed Hasaart KAL, et al. 2022. iScience. 25:103736. PubMed Rai S, et al. 2022. Nat Commun. 13:5346. PubMed Okumura T, et al. 2019. Stem Cell Res Ther. 10:185. PubMed
RRID: AB_10644012 (BioLegend Cat. No. 348701)
Distribution: CD3 is expressed on all T lymphocytes. CD14 is expressed on monocytes, macrophages, neutrophils, and eosinophils. CD19 and CD20 are expressed on B lymphocytes. CD56 is expressed on activated and resting NK lymphocytes.
Cell Type: Dendritic cells, Eosinophils, Macrophages, Mesenchymal Stem Cells, Monocytes, Neutrophils, T cells
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey. 2. Olweus J, et al. 1997. P. Natl. Acad. Sci. USA 94:12551. 3. Banchereau J, et al. Eds. 1995. Advances in Experimental Medicine and Biology. New York. 4. Markowicz S, et al. 1990. J. Clin. Invest. 85:955. 5. Freudenthal P, et al. 1990. P. Natl. Acad. Sci. USA 87:7698. 6. Macdonald K, et al. 2002. Blood 100:4512. 7. Piccioli D, et al. 2007. Blood 109:5371.
Gene ID: 9159309314684929
UniProt: View information about Lineage on UniProt.org
Clone: UCHT1; HCD14; HIB19; 2H7; HCD56;
Regulatory Status: RUO
Isotype: Mouse IgG1, κ/Mouse IgG2b, κ