Biolegend, 348801, FITC anti-human Lineage Cocktail (CD3, CD14, CD16, CD19, CD20, CD56), 50tests

This anti-Human Lineage Cocktail is optimized for the detection of human peripheral blood T cells, B cells, NK cells, monocytes, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells and/or basophils. This cocktail is composed of CD3, CD14, CD16, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD16 is expressed on NK cells, activated monocytes/macrophages, and neutrophils; CD19 and CD20 are on B cells; CD56 is located on NK cells.
50tests
Verified Reactivity: Human
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: View more applications data for this product in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey. (FC) Doherty TA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:577. PubMed.
Product Citations: Shafiei–Jahani P, et al. 2020. Nat Commun. 3.734722222. PubMed Kfoury Y, et al. 2021. Cancer Cell. 39:1464. PubMed van der Sluis RM, et al. 2022. EMBO J. 41:e109622. PubMed Doherty T, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:577. PubMed Laustsen A, et al. 2021. Elife. 10:. PubMed Lal A, et al. 2021. Nat Commun. 1507:12. PubMed Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed Imai Y, et al. 2021. JID Innov. 1:100003. PubMed Delaney C, et al. 2021. EMBO Mol Med. 13:e12889. PubMed Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed Zavidij O, et al. 2020. Nat Cancer. 0.384027778. PubMed Levy Y, et al. 2021. iScience. 24:102711. PubMed Zhang JA, et al. 2020. Front Immunol. 11:518. PubMed Feldman S, et al. 2017. Clin Exp Allergy. 47:457. PubMed Wiedemann A, et al. 2020. Nat Commun. 3.048611111. PubMed Serr I, et al. 2016. Nat Commun. 7:10991. PubMed Shevchenko JA, et al. 2020. Oncol Rep. 43:671. PubMed Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed
RRID: AB_10612570 (BioLegend Cat. No. 348801)
Distribution: T cells, B cells, NK cells, monocytes/macrophages, neutrophils
Cell Type: B cells, Dendritic cells, Macrophages, Mesenchymal Stem Cells, Monocytes, Neutrophils, NK cells, T cells
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Gene ID: 91522149309314684929
UniProt: View information about Lineage on UniProt.org
Clone: UCHT1; HCD14; 3G8; HIB19; 2H7; HCD56;
Regulatory Status: RUO
Other Names: Lin 1
Isotype: Mouse IgG1, κ/Mouse IgG2b, κ