Biolegend, 348902, Purified anti-human Galectin-9 Antibody, 100μg

Galectin-9 is a mammalian lectin with a molecular weight around 50 kD. It is a member of the β-galactoside-binding family. With two conserved carbohydrate recognition domains (CRDs), galectin-9 binds small β-galactosides as well as complex glycoconjugates.  HAVCR2/TIM3 has been reported as one of its ligands. Galectin-9 may be retained intracellularly or transported to the cell surface where it can be cleaved to generate a soluble form. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts, and thymus epithelial cells. It can be induced by cytokines in various cell types and is involved in cell aggregation, adhesion, chemotaxis, and apoptosis; galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant peptide from C-terminus of Galectin-9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested IHC-P - Verified FC, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: cell surface staining for flow cytometry1 and blocking of TIM-3 binding to galectin-91.
Application References(PubMed link indicates BioLegend citation): Klibi J, et al. 2009. Blood 113:1957. (FC, Block) Sada-Ovalle I, et al. 2012. J. Immunol. 189:5896. PubMed
Product Citations: Wang H, et al. 2022. Front Immunol. 13:852436. PubMed Lee M, et al. 2022. Nat Commun. 13:1157. PubMed Sada-Ovalle I, et al. 2012. J Immunol. 189:5896. PubMed Guo Q, et al. 2022. iScience. 25:105329. PubMed Chakraborty A, et al. 2022. Methods Mol Biol. 2442:565. PubMed
RRID: AB_10612753 (BioLegend Cat. No. 348902)
Structure: Member of the β-galactoside-binding lectin family. Molecular weight around 50 kD. Binds small β-galactosides as well as complex glycoconjugates with two conserved carbohydrate recognition domains (CRDs).
Distribution: Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells.
Function: Cell aggregation, adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, and induction of regulatory T cells.
Ligand/Receptor: HAVCR2/TIM3
Cell Type: Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: Adhesion Molecules, Immune Checkpoint Receptors
Antigen References: 1. Seki M, et al. 2008. Clin. Immunol. 127:78. 2. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221. 3. Zhu C, et al. 2005. Nat. Immunol. 6:1245. 4. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916.
Gene ID: 3965
UniProt: View information about Galectin-9 on UniProt.org
Clone: 9M1-3
Regulatory Status: RUO
Other Names: Lgals9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, Ecalectin
Isotype: Mouse IgG1, κ