Biolegend, 348905, PE anti-human Galectin-9 Antibody, 25tests

Galectin-9 is a mammalian lectin with a molecular weight around 50 kD. It is a member of the β-galactoside-binding family. With two conserved carbohydrate recognition domains (CRDs), galectin-9 binds small β-galactosides as well as complex glycoconjugates.  HAVCR2/TIM3 has been reported as one of its ligands. Galectin-9 may be retained intracellularly or transported to the cell surface where it can be cleaved to generate a soluble form. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts, and thymus epithelial cells. It can be induced by cytokines in various cell types and is involved in cell aggregation, adhesion, chemotaxis, and apoptosis; galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant peptide from C-terminus of Galectin-9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality testedFC, - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: cell surface staining for flow cytometry1 and blocking of TIM-3 binding to galectin-91.
Application References(PubMed link indicates BioLegend citation): Klibi J, et al. 2009. Blood 113:1957. (FC, Block) Sada-Ovalle I, et al. 2012. J. Immunol. 189:5896. PubMed
Product Citations: Harwood N, et al. 2016. J Leukoc Biol. 99: 495 - 503. PubMed Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed Miko E, et al. 2013. PLoS One. 8:71811. PubMed Nielsen M, et al. 2016. Rheumatology. 55: 1871 - 1879. PubMed Qiu Y, et al. 2012. PLoS One. 8:e1002984. PubMed Arenas EJ, et al. 2021. Nat Commun. 12:1237. PubMed Meggyes M, et al. 2021. Biomolecules. 11:. PubMed
RRID: AB_10613284 (BioLegend Cat. No. 348905) AB_10613466 (BioLegend Cat. No. 348906)
Structure: Member of the β-galactoside-binding lectin family. Molecular weight around 50 kD. Binds small β-galactosides as well as complex glycoconjugates with two conserved carbohydrate recognition domains (CRDs).
Distribution: Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells.
Function: Cell aggregation, adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, and induction of regulatory T cells.
Ligand/Receptor: HAVCR2/TIM3
Cell Type: Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: Adhesion Molecules, Immune Checkpoint Receptors
Antigen References: 1. Seki M, et al. 2008. Clin. Immunol. 127:78. 2. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221. 3. Zhu C, et al. 2005. Nat. Immunol. 6:1245. 4. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916.
Gene ID: 3965
UniProt: View information about Galectin-9 on UniProt.org
Clone: 9M1-3
Regulatory Status: RUO
Other Names: Lgals9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, Ecalectin
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.