Biolegend, 349106, PE anti-human CD235a (Glycophorin A) Antibody, 100μg

CD235a (Glycophorin A) is member of the glycophorin A family. It is a type I sialoglycoprotein with a molecular weight of 10 kD, present in the cell membrane as a homodimer. Glycophorin A is expressed by erythroid precursors and erythrocytes.  It carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. Glycophorin A binds siglec 5, the erythrocyte binding antigen (EBA-175) of P. falciparum and some viruses, including influenza virus and hepatitis A virus.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Mason D, et al. Eds. 2002. Leucocyte Typing VII:White Cell Differentiation Antigens. Oxford University Press. (FC)
Product Citations: Tamaoki N, et al. 2023. Cell Rep Methods. 3:100460. PubMed Calcagno DM, et al. 2020. Sci Immunol. 5: . PubMed Liao J, et al. 2023. Nat Commun. 14:207. PubMed Dickerson KM, et al. 2022. Blood Cancer Discov. 3:240. PubMed Carraro G, et al. 2021. Nat Med. 27:806. PubMed Shaham L, et al. 2015. Blood. 125:1292. PubMed Carraro G, et al. 2020. Am J Respir Crit Care Med. 202:1540. PubMed Fan X, et al. 2021. Genome Biol. 22:195. PubMed Liu S, et al. 2021. Mol Ther. 29:1918. PubMed Celik H, et al. 2021. Cancer Discov. 11:3126. PubMed Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed Sakamoto S, et al. 2015. PLoS One. 10: e0139915. PubMed Rodriguez A, et al. 2021. Cell Stem Cell. 28:33. PubMed Cai J, et al. 2014. PLoS One. 9:114768. PubMed
RRID: AB_10641707 (BioLegend Cat. No. 349105) AB_10640739 (BioLegend Cat. No. 349106)
Structure: Member of the glycophorin A family; type I protein with a molecular weight of 10 kD; present in the membrane as homodimer
Distribution: Erythrocytes and erythroid precursors
Function: Possible inhibitor of hemagglutination and hemolysis; carries the antigen determinants for the MNS blood groups
Ligand/Receptor: Siglec 5, erythrocyte binding antigen (EBA-175) of P. falciparum, receptor for the influenza virus, hepatitis A virus
Cell Type: Erythrocytes
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Reid ME. 2009. Immunohematology 25:95. 2. Palacajornsuk P. 2006. Immunohematology 22:171. 3. Pasvol G. 2003. Trends Parasitol. 19:430. 4. Takakuwa Y. 2001. Curr. Opin. Hematol. 8:80.
Gene ID: 2993
UniProt: View information about CD235a on UniProt.org
Clone: HI264
Regulatory Status: RUO
Workshop: VII 70312
Other Names: PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, Glycophorin A, GYPA
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.