Biolegend, 349301, Purified anti-human TCR Vβ5 Antibody, 25μg

Vβ5 T cell receptor (TCR Vβ5) is a variant of the TCR β chain which is heterodimerized with TCR α chain and expressed on a subset of αβ + T cells. TCR α/β recognizes peptides presented by the MHC molecules and initiates T cell mediated immune responses. Patients with multiple sclerosis present an expansion of Vβ5.2 + /5.3 + autoreactive T cells.
25μg
Verified Reactivity: Human
Reported Reactivity: Pigtailed Macaque
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Labrecque N, et al. 1993. J. Exp. Med. 177:1735. (FC) Oh S, et al. 2004. Cancer Res. 64:2610. (FC) Wilson JDK, et al. 1998. Eur. J. Immunol. 28:1172. (FC) Patel MR, et al. 2010. Cancer Res. 70:10141. PubMed Han JA, et al. 2014. Nanomedicine. 10:561. PubMed
Product Citations: Patel M, et al. 2010. Cancer Res. 70:10141. PubMed Li F, et al. 2022. Nat Commun. 13:4334. PubMed Han J, et al. 2014. Nanomedicine. 10:561. PubMed
RRID: AB_10643416 (BioLegend Cat. No. 349301)
Structure: Immunoglobulin superfamily, associates with TCR Vα chain and CD3 complex
Distribution: Subset of TCR αβ+ T cells
Function: Recognition of peptides presented by MHC molecules, responsible for T cell mediated immune response
Ligand/Receptor: MHC-peptide complex
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Kuchroo VK, et al. 2002. Annu. Rev. Immunol. 20:101. 2. Venturi V, et al. 2008. Nat. Rev. Immunol. 8:231. 3. Matsumoto Y, et al. 2003. J. Immunol. 170:4846. 4. Killestein J, et al. 2002. Ann. Neurol. 51:467.
Gene ID: 6957
UniProt: View information about TCR Vbeta5 on UniProt.org
Clone: MH3-2
Regulatory Status: RUO
Other Names: T cell receptor V beta 5
Isotype: Mouse IgG2a, κ