Biolegend, 350014, Brilliant Violet 421™ anti-human CD134 (OX40) Antibody, 100tests

CD134, also known as OX40 and TNFRSF4, is a 50 kD type I transmembrane glycoprotein. It is a member of the TNF receptor family. OX40 is expressed on activated T lymphocytes including Th1, Th2, Th17, and Treg cells. The interaction of OX40 with OX40L results in B cell proliferation and antibody secretion, regulation of primary T cell expansion, and T cell survival. OX40 influences the size of the T cell memory pool and regulation of CD4 + T cell tolerance.
100tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HTLV 1-transformed HUT 102 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, immunohistochemical staining2,3of paraffin embedded7 and frozen tissue sections, ELISA4, and functional assay5. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).
Application References(PubMed link indicates BioLegend citation): Latza U, et al. 1994. Eur. J. Immunol. 24:677. (WB, IP) Durkop H, et al. 1995. Brit. J. Haematol. 91:927. (IHC) Durkop H, et al. 1997. Brit. J. Haematol. 98:863. (IHC) Willett B, et al. 2007. J. Virol. 81:9665. (ELISA) Li M and Zhang Y. et al. 2005. Cell. Mol. Immunol. 2:467. (FA) Gloviczki ML, et al. 2012. Clin. J. Am. Soc. Nephrol. 8:546. PubMed Domingos PL, et al. 2012. An. Bras. Dermatol. 87:851. (IHC)
Product Citations: Robinson A, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:1115. PubMed Jung JH, et al. 2021. Nat Commun. 12:4043. PubMed Nguyen J, et al. 2021. Mol Syst Biol. 17:e10560. PubMed
RRID: AB_10895764 (BioLegend Cat. No. 350013) AB_2564184 (BioLegend Cat. No. 350014)
Structure: 50 kD, TNF receptor family
Distribution: Activated T cells
Function: Receptor for OX40 ligand, co-stimulatory signal for T cell proliferation and survival.
Ligand/Receptor: OX40L (CD252)
Cell Type: T cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Smith CA, et al. 1994. Cell. 76:959. 2. Chen AL, et al. 1999. Immunity. 11:689. 3. Croft M. 2010. Annu. Rev. Immunol. 28:57. 4. Withers DR, et al. 2009. J. Immunol. 183:5079. 5. Klinger M, et al. 2009. J. Immunol. 182:4581.
Gene ID: 7293
UniProt: View information about CD134 on UniProt.org
Clone: Ber-ACT35 (ACT35)
Regulatory Status: RUO
Other Names: ACT35 antigen, tumor necrosis factor receptor superfamily member 4 (TNFRSF4)
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.