Biolegend, 350405, APC anti-human CD151 (PETA-3) Antibody, 25tests

CD151, also known as PETA-3, is a 29 kD protein member of the tetraspanin family (TM4SF). As other members in the family, PETA-3 is composed of two extracellular loops (one larger than the other), four transmembrane domains, and two cytoplasmic tails. Also, as other members in the family, CD151 is involved in cell adhesion, migration, and tumor cell metastasis. PETA-3 is expressed by many different cell types such as immature hematopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells, and vascular endothelium. On the cell surface, PETA-3 associates with CD9, CD181, and the integrins α3β1, α5β1, and α6β4.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human epidermoid carcinoma cell line HEp-3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vivo inhibition of metastasis of HEp-3 cells1, Western blotting1, immunocytochemical staining of HEp-3 cells1, and CD151-transfected COS cells fixed with 0.25% glutaraldehyde.
Application References(PubMed link indicates BioLegend citation): Testa JE, et al. 1999. Cancer Res. 59:3812. (Block, ICC, WB)
Product Citations: Yunusova NV, et al. 2023. J Pers Med. 13:. PubMed Yunusova NV, et al. 2022. Int J Mol Sci. 24: . PubMed Yunusova NV, et al. 2021. Asian Pac J Cancer Prev. 22:861. PubMed
RRID: AB_10661726 (BioLegend Cat. No. 350405) AB_10661727 (BioLegend Cat. No. 350406)
Structure: Member of the tetraspanin family (TM4SF), 29 kD protein, consist of two extracellular loop, four transmembrane domains and two cytoplasmic tails
Distribution: Immature hematopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells, vascular endothelium
Function: Cell adhesion, migration
Interaction: CD9, CD181, integrins α3β1, α5β1 and α6β4
Cell Type: Epithelial cells, Hematopoietic stem and progenitors, Megakaryocytes, Mesenchymal Stem Cells, Platelets
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zöller M. 2009. Nat. Rev. Cancer 9:40. 2. Yañez-Mó M, et al. 2008. Blood 112:3217. 3. Nishiuchi R, et al. 2005. Proc. Natl. Acad. Sci. USA 102:1939.
Gene ID: 977
UniProt: View information about CD151 on UniProt.org
Clone: 50-6
Regulatory Status: RUO
Other Names: Platelet-endothelial tetraspan antigen 3, GP27, Membrane glycoprotein SFA-1, Tetraspanin-24, Tspan-24
Isotype: Mouse IgG1, κ