Biolegend, 350814, PerCP/Cyanine5.5 anti-human CD325 (N-Cadherin) Antibody, 100tests

CD325 (N-cadherin) is a 130 kD, single pass transmembrane protein. Its extracellular region consists of five EC domains and has one cytoplasmic domain. N-cadherin is involved in organogenesis and maintenance of organ architecture by contributing to the sorting of heterogeneous cell types and in the cell adhesion needed to form tissues. N-cadherin is expressed by stem cells, myeloblasts, endothelial cells, and fibroblasts, and also is expressed in neural and muscle tissues and some types of carcinoma cells. CD325 associates with the cytoskeleton trough catenin proteins.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human N-cadherin extracellular domain
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The mAb 8C11 recognizes the amino acids 92–593 of CD325, located between the extracellular cadherin structural domain (EC) 3 and 4. Additional reported applications (for the relevant formats) include: immunofluorescence1,3,6, motility inhibition of N-cadherin-expressing cells2, and Western blot2,4.
Application References(PubMed link indicates BioLegend citation): Navarro P, et al. 1998. J. Cell Biol. 140:1475. (IF) Kim JB. 2000. J. Cell Biol. 151:1193. (Block, WB) Puch S, et al. 2001. J. Cell. Sci. 114:1567. (IF) Wahl JK. 3rd, et al. 2003. J. Biol. Chem. 278:17269. (WB) Wein F, et al. 2010. Stem. Cell Res. 4:129. (FC) Jaggi M, et al. 2002. Cell. Commun. Adhes. 9:103. (IF)
Product Citations: Chirshev E, et al. 2020. Mol Oncol. . PubMed
RRID: AB_2562685 (BioLegend Cat. No. 350813) AB_2562686 (BioLegend Cat. No. 350814)
Structure: Member of cadherin family, Single pass transmembrane protein, extracellular region consist of five EC domains, one cytoplasmic domain, 130 kD
Distribution: Stem cells, myeloblasts, endothelial cells, fibroblasts, neural and muscle tissues, some types of carcinoma cells
Function: Embryonic development, maintain tissue architecture, cell sorting and cell adhesion in tissues, promote motility
Interaction: Catenins
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Colomiere M, et al. 2009. Brit. J. Cancer 100:134. 2. Yan W, et al. 2010. J. Biol. Chem. 285:14042. 3. Mosnier JF, et al. 2009. Mod. Pathol. 22:182. 4. Gao L, et al. 2010. Stem Cells 28:564.
Gene ID: 1000
UniProt: View information about CD325 on UniProt.org
Clone: 8C11
Regulatory Status: RUO
Other Names: Cadherin-2, Neural cadherin
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.