Biolegend, 351304, PE anti-human CD127 (IL-7Rα) Antibody, 100tests

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD127
Formulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported (for the relevant formats) application: proteogenomics1.
Application References(PubMed link indicates BioLegend citation): Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations: Wang X, et al. 2019. Cell Res. 29:787. PubMed Gkika E, et al. 2023. NPJ Precis Oncol. 7:24. PubMed Wang P, et al. 2023. Front Immunol. 13:1104329. PubMed Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed Richardson SE, et al. 2021. STAR Protocols. 2(2):100420. PubMed Kollis PM, et al. 2022. Front Immunol. 13:850226. PubMed Schroder K, et al. 2012. Proc Natl Acad Sci U S A. 109:944. PubMed Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed García-Ríos E, et al. 2022. Biomedicines. 10:. PubMed Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed Carnevale J, et al. 2022. Nature. 609:174. PubMed Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed Yang L, et al. 2020. Genes Dis. 7:128. PubMed Su X, et al. 2022. J Transl Med. 20:378. PubMed Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed Fang F, et al. 2021. Cell Rep. 37:109981. PubMed NULL, et al. 2022. Cell. 185:916. PubMed Rönnberg E, et al. 2019. Front Immunol. 1.361805556. PubMed Keck S, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 12(2):507-545. PubMed
RRID: AB_10719960 (BioLegend Cat. No. 351303) AB_2564136 (BioLegend Cat. No. 351340) AB_10720185 (BioLegend Cat. No. 351304)
Structure: Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand/Receptor: IL-7
Cell Type: B cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503. 3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610. 4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403. 5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707. 6. Liu W, et al. 2006. J. Exp. Med. 203:1701.
Gene ID: 3575
UniProt: View information about CD127 on UniProt.org
Clone: A019D5
Regulatory Status: RUO
Other Names: IL-7 receptor α chain, IL-7Rα
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.