Biolegend, 351342, APC anti-human CD127 (IL-7Rα) Antibody, 100μg

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD127
Formulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported (for the relevant formats) application: proteogenomics1.
Application References(PubMed link indicates BioLegend citation): Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations: Avital G, et al. 2022. Cell Rep. 41:111477. PubMed Sudmeier LJ, et al. 2022. Cell Rep Med. 3:100620. PubMed Cui B, et al. 2023. Int Immunopharmacol. 114:109541. PubMed Ye L, et al. 2022. Cell Metab. 34:595. PubMed Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Vandooren J, et al. 2021. Front Immunol. 12:701739. PubMed Hixon JA, et al. 2020. Leukemia. 34:35. PubMed Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed Christophersen A, et al. 2016. J Immunol. 196: 2819 - 2826. PubMed Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed Kim SY, et al. 2018. Invest Ophthalmol Vis Sci. 59:5693. PubMed Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed Grivas A, et al. 2022. Front Immunol. 13:964274. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Goods BA, et al. 2021. JCI Insight. 6: . PubMed Shen C, et al. 2021. Front Immunol. 12:680055. PubMed Batikan O, et al. 2022. J Coll Physicians Surg Pak. 32:1004. PubMed Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed Imura Y, et al. 2020. JCI Insight. :5. PubMed Schielke L, et al. 2022. Front Immunol. 13:916701. PubMed Nabatanzi R, et al. 2021. BMC Immunol. 22:59. PubMed Grigoriou M, et al. 2021. Cancer Immunol Res. 9:726. PubMed Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed Li J, et al. 2017. Toxicology. 378:17. PubMed Sehgal R, et al. 2022. Front Immunol. 13:828949. PubMed Wang L, et al. 2018. Oncol Lett. 15:8635. PubMed Rochman Y, et al. 2015. PLoS One. 10:122198. PubMed Gamradt S, et al. 2021. iScience. 24:103312. PubMed
RRID: AB_10900814 (BioLegend Cat. No. 351315) AB_2564137 (BioLegend Cat. No. 351342) AB_10900804 (BioLegend Cat. No. 351316)
Structure: Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand/Receptor: IL-7
Cell Type: B cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503. 3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610. 4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403. 5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707. 6. Liu W, et al. 2006. J. Exp. Med. 203:1701.
Gene ID: 3575
UniProt: View information about CD127 on UniProt.org
Clone: A019D5
Regulatory Status: RUO
Other Names: IL-7 receptor α chain, IL-7Rα
Isotype: Mouse IgG1, κ