Biolegend, 351402, Purified anti-human CD65 Antibody, 100μg

CD65, also referred to as ceramide-dodecasaccharide or type II fucoganglioside, is a fucosylated carbohydrate antigen which serves as a ligand for CD62E, commonly known as E-selectin. A key difference between CD65 and CD65s is that CD65 does not have a terminal sialic acid, although the rest of their structures are identical. CD65 is expressed on most myeloid cells during development, highly on granulocytes and weakly on monocytes in peripheral blood, and plays a role in cell adhesion. It has been identified as a key adhesive ligand that plays a significant role in the extravascular infiltration of cells associated with acute myeloid leukemia (AML).
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: THP-1 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Lund­-Johansen F, et al. 1992. J Immunol. 148:3221-9. Bordessoule D, et al. 1993. Br J Haematol. 83:370-83. Kniep B, et al. 1996. J. Biochem 119:456-62.
RRID: AB_3675072 (BioLegend Cat. No. 351402)
Structure: The structure of CD65 is defined as Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide.
Distribution: Most myeloid cells during development, highly on granulocytes and weakly on monocytes in peripheral blood.
Function: Cell adhesion
Ligand/Receptor: Serve as a ligand for CD62E, also known as E-selection
Cell Type: Granulocytes, Leukocytes, Monocytes
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules
Antigen References: Joziasse DH, et al. 1989. J. Biol. Chem. 264:14290-7.
Gene ID: NA
Clone: VIM8
Regulatory Status: RUO
Other Names: Ceramide dodecasacharide, VIM2
Isotype: Mouse IgM, κ