Biolegend, 351701, Purified anti-human TCR Vα7.2 Antibody, 25μg

The 3C10 antibody recognizes the Vα7.2 T cell antigen receptor (TCR) α-chain segment which, joined with the Jα33 segment, constitutes an invariant TCR that is a characteristic of the mucosal-associated invariant T cells (MAIT cells). MAIT cells are restricted by a nonpolymorphic class Ib major histocompatibility complex (MHC) molecule, MHC-related molecule 1 (MR1). MAIT cells are present in human blood (1-8% of T cells), mesenteric lymph nodes, liver, and intestinal mucosa.  MAIT cells play a role in detecting and fighting off microbial infections.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant TCR
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2 segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.
Application References(PubMed link indicates BioLegend citation): Martin E, et al. 2009. PLoS Biol. 7:525. Wakao H, et al. 2013. Cell Stem Cell 12:1. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Shaler C, et al. 2017. Cancer Immunol Immunother. 10.1007/s00262-017-2050-7. PubMed Souter MNT, et al. 2022. J Exp Med. 219:. PubMed Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed Wakao H, et al. 2013. Cell Stem Cell. 12:1. PubMed Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed Ye G, et al. 2021. Stem Cell Reports. 16:926. PubMed Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed Marrero I, et al. 2020. Alcohol Clin Exp Res. 44:1061. PubMed Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed Jiang H, et al. 2022. Clin Transl Med. 12:e730. PubMed Nicol B,et al. 2017. J Autoimmun. . 10.1016/j.jaut.2017.10.005. PubMed
RRID: AB_10900258 (BioLegend Cat. No. 351702)
Structure: Invariant T cell antigen receptor (TCR) α-chain (iTCRa), composed of the invariant α-chain variable region 7.2 (iVα7.2) segment in humans
Distribution: Mucosal-associated invariant T (MAIT) cells and conventional T cells
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Le Bourhis L, et al. 2010. Nat. Immunol. 11:701.
Gene ID: 28692
UniProt: View information about TCR Valpha7.2 on UniProt.org
Clone: 3C10
Regulatory Status: RUO
Other Names: T cell antigen receptor Vα7.2, TRAV1-2, T cell receptor alpha variable 1-2
Isotype: Mouse IgG1, κ