Biolegend, 351708, APC anti-human TCR Vα7.2 Antibody, 100tests

The 3C10 antibody recognizes the Vα7.2 T cell antigen receptor (TCR) α-chain segment which, joined with the Jα33 segment, constitutes an invariant TCR that is a characteristic of the mucosal-associated invariant T cells (MAIT cells). MAIT cells are restricted by a nonpolymorphic class Ib major histocompatibility complex (MHC) molecule, MHC-related molecule 1 (MR1). MAIT cells are present in human blood (1-8% of T cells), mesenteric lymph nodes, liver, and intestinal mucosa.  MAIT cells play a role in detecting and fighting off microbial infections.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant TCR
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2 segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.
Application References(PubMed link indicates BioLegend citation): Martin E, et al. 2009. PLoS Biol. 7:525. Wakao H, et al. 2013. Cell Stem Cell 12:1. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Duhalde Vega M, et al. 2022. Sci Adv. 8:eabn6545. PubMed Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Yero A, et al. 2022. J Virol. 96:e0025522. PubMed Lejon K, et al. 2022. Scand J Immunol. 96:e13190. PubMed Wakao H, et al. 2013. Cell Stem Cell. 12:1. PubMed Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed Sattler A, et al. 2019. J Immunol. 203:2970. PubMed Hinks TSC, et al. 2019. Cell Rep. 28:3249. PubMed Ben Youssef G, et al. 2018. J Exp Med. 215:459. PubMed Sundström P, et al. 2015. J Immunol. 195: 3472 - 3481. PubMed Kang S, et al. 2016. PLoS One. 10: 0004832. PubMed Dias J, et al. 2016. J Leukoc Biol. 100: 233 - 240. PubMed Cottineau J, et al. 2017. J Clin Invest. 127:1991. PubMed McWilliam H, et al. 2016. Nat Immunol. 17: 531-537. PubMed Li H, et al. 2022. Cell Death Dis. 13:849. PubMed Keller A, et al. 2017. Nat Immunol. 18:402-411. PubMed Gracey E, et al. 2016. Ann Rheum Dis. 75(12):2124-2132. PubMed Valestrand L, et al. 2022. Am J Pathol. . PubMed Leng T, et al. 2019. Cell Rep. 28:3077. PubMed Wilgenburg B, et al. 2016. Nat Commun. 7:11653. PubMed Ling L, et al. 2016. Sci Rep. 6:20358. PubMed Cosgrove C, et al. 2013. Blood. 121:951. PubMed Cho Y, et al. 2014. J Immunol. 193:3891. PubMed
RRID: AB_10933430 (BioLegend Cat. No. 351707) AB_10933246 (BioLegend Cat. No. 351708)
Structure: Invariant T cell antigen receptor (TCR) α-chain (iTCRa), composed of the invariant α-chain variable region 7.2 (iVα7.2) segment in humans
Distribution: Mucosal-associated invariant T (MAIT) cells and conventional T cells
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Le Bourhis L, et al. 2010. Nat. Immunol. 11:701.
Gene ID: 28692
UniProt: View information about TCR Valpha7.2 on UniProt.org
Clone: 3C10
Regulatory Status: RUO
Other Names: T cell antigen receptor Vα7.2, TRAV1-2, T cell receptor alpha variable 1-2
Isotype: Mouse IgG1, κ