Biolegend, 351724, Biotin anti-human TCR Vα7.2 Antibody, 100μg

The 3C10 antibody recognizes the Vα7.2 T cell antigen receptor (TCR) α-chain segment which, joined with the Jα33 segment, constitutes an invariant TCR that is a characteristic of the mucosal-associated invariant T cells (MAIT cells). MAIT cells are restricted by a nonpolymorphic class Ib major histocompatibility complex (MHC) molecule, MHC-related molecule 1 (MR1). MAIT cells are present in human blood (1-8% of T cells), mesenteric lymph nodes, liver, and intestinal mucosa.  MAIT cells play a role in detecting and fighting off microbial infections.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant TCR
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2 segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.
Application References(PubMed link indicates BioLegend citation): Martin E, et al. 2009. PLoS Biol. 7:525. Wakao H, et al. 2013. Cell Stem Cell 12:1. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Halkias J, et al. 2019. J Clin Invest. 130:3562. PubMed
RRID: AB_2566057 (BioLegend Cat. No. 351724)
Structure: Invariant T cell antigen receptor (TCR) α-chain (iTCRa), composed of the invariant α-chain variable region 7.2 (iVα7.2) segment in humans
Distribution: Mucosal-associated invariant T (MAIT) cells and conventional T cells
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Le Bourhis L, et al. 2010. Nat. Immunol. 11:701.
Gene ID: 28692
UniProt: View information about TCR Valpha7.2 on UniProt.org
Clone: 3C10
Regulatory Status: RUO
Other Names: T cell antigen receptor Vα7.2, TRAV1-2, T cell receptor alpha variable 1-2
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.