Biolegend, 351802, Purified anti-human CD221 (IGF-1R) Antibody, 100μg

CD221, also known as insulin-like growth factor 1 receptor (IGF1R), is a 135 kD transmembrane receptor. It is widely expressed on lymphoid and myeloid cells as well as non-haematopoietic cells. CD221 is a tyrosine kinase receptor and the ligands for CD221 are insulin-like growth factor 1 and 2 (IGF-1 and IGF-2), the polypeptide hormones similar in molecular structure to insulin. IGF-1/2 binding activates downstream PI3K and MAPK pathways and plays an important role in cell growth. Recent studies reveal its function of recruiting immune cells during tissue repair.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: IGF-1 receptor purified from human placentas
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC, IP, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone has been published as 1H7. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, immunohistochemical staining of frozen tissue sections2, and blocking functions2-5. This product is offered for sale and sold pursuant to a license to seller by City of Hope and may be used only as a research reagent. Use for any other purpose is expressly not authorized.
Application References(PubMed link indicates BioLegend citation): Li SL, et al. 1993. Biochem. Bioph. Res. Co. 196:92. (WB IP) Li SL, et al. 2000. Cancer Immunol. Immun. 49:243. (IHC Block) Ye JJ, et al. 2003. Horm. Metab. Res. 35:836. (Block) Kusada Y, et al. 2008. J. Biochem. 143:9. (Block) Ohtani M, et al. 2009. Biosci. Trends. 3:131. (Block)
RRID: AB_10896950 (BioLegend Cat. No. 351802)
Structure: IGF-1R contains 2 α subunits and 2 β subunits, 135 kD
Distribution: Haematopoietic cells and non-haematopoietic cells
Function: Cell growth and immune cells recruitment
Ligand/Receptor: IGF-1, IGF-2
Biology Area: Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Baudler S, et al. 2005. J. Immunol. 174:5516. 2. Sharp LL, et al. 2005. Nat. Immunol. 6:73.
Gene ID: 3480
UniProt: View information about CD221 on UniProt.org
Clone: 1H7/CD221
Regulatory Status: RUO
Other Names: Insulin-like growth factor 1 receptor (IGF1R)
Isotype: Mouse IgG1, κ