Biolegend, 352004, PE anti-human CD170 (Siglec-5) Antibody, 100tests

CD170, also known as Siglec-5, is a single pass transmembrane protein member of the immunoglobulin superfamily. On the cell surface, CD170 forms a 140 kD dimer. The cytoplasmic domain of Siglec-5 contains two ITIM motifs that recruit the tyrosine-phosphatases SHP-1 and SHP-2 after tyrosine-phosphorylation, which in turn results in the inhibition of cell signaling. Siglec-5 is expressed by granulocytes, monocytes/macrophages, subsets of lymphocytes, and a subset of activated dendritic cells. Siglec-5 binds α2,3- and α2,6-linked sialic acid as well as glycophorin A, and is involved in cell adhesion.
100tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Bonobo, Gorilla
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Extracellular region of Siglec-5
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The 1A5 antibody cross-reacts with Siglec-144. Additional reported applications (for the relevant formats) include: ELISA2. Clone 1A5 cross-reacts to Gorilla2
Application References(PubMed link indicates BioLegend citation): Cornish AL, et al. 1998. Blood 92:2123. Nguyen DH, et al. 2006. P. Natl. Acad. Sci. USA 103:7765. (FC, ELISA) Connolly NP, et al. 2002. Br. J. Haematol. 119:221. Angata T, et al. 2006. FASEB J. 20:1964. Avril T, et al. 2005. J. Biol. Chem. 280:19843.
Product Citations: Ghadially H, et al. 2013. Eur J Immunol. 3006:43. PubMed Tecle E, et al. 2019. J Biol Chem. 294:11910. PubMed Jobe O, et al. 2016. J Leukoc Biol. 99: 1089 - 1106. PubMed
RRID: AB_10896433 (BioLegend Cat. No. 352003) AB_10895766 (BioLegend Cat. No. 352004)
Structure: Member of the immunoglobulin superfamily, single pass transmembrane protein, forms a 140 kD dimer; cytoplasmic domain contains two ITIM motifs
Distribution: Granulocytes, monocytes/macrophages, subsets of lymphocytes, and activated dendritic cells
Function: Inhibitory receptor, cell adhesion
Interaction: SHP-1, SHP-2
Ligand/Receptor: α2,3- and α2,6-linked sialic acid, glycophorin A
Cell Type: B cells, Dendritic cells, Granulocytes, Lymphocytes, Macrophages, Monocytes
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Siglec Molecules
Antigen References: 1. Soto PC, et al. 2010. J. Immunol. 184:4185. 2. Carlin AF, et al. 2009. J. Exp. Med. 206:1691. 3. Zhuravleva MA. 2008. J. Mol. Biol. 375:437.
Gene ID: 8778
UniProt: View information about CD170 on UniProt.org
Clone: 1A5
Regulatory Status: RUO
Workshop: VII 70443
Other Names: OBBP2, CD33L2, OB-BP2
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.