Biolegend, 352206, APC anti-human CD207 (Langerin) Antibody, 100tests

CD207, also known as Langerin, is a 40 kD type II transmemberane cell glycoprotein which belongs to C-type lectin with mannose binding specificity. It is predominantly expressed on Langerhans cells and induces the formation of Birbeck granules, the Langerhans cell hallmark organelle. It is also found on several other subtypes of dendritic cells, such as dermal CD103-positive dendritic cells and splenic CD8-positive dendritic cells. Langerin is generally thought to be involved in antigen processing. Recently, it has been found that HIV captured by Langerin was internalized into Birbeck granule and degraded, which results in inhibition of HIV-1 infection and subsequent transmission.
100tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Primary human Langerhans cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported application (for the relevant formats) includes: blocking the binding of HIV-1 to Langerhans cells1.
Application References(PubMed link indicates BioLegend citation): Witte LD, et al. 2007. Nat. Med. 13:367. (Block)
Product Citations: Xu Y, et al. 2021. Cell Reports. 36(6):109524. PubMed
RRID: AB_2561589 (BioLegend Cat. No. 352205) AB_2561590 (BioLegend Cat. No. 352206)
Structure: 40 kD glycoprotein
Distribution: Langerhans cells, dermal CD103-positive dendritic cells, and splenic CD8-positive dendritic cells
Function: Binding and processing antigen; capturing, internalizing and degrading HIV
Ligand/Receptor: Antigen containing mannose, N-acetyl glucosamine, and fucose
Cell Type: Dendritic cells, Langerhans cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Valladeau J, et al. 2000. Immunity 12:71. 2. Mc Dermott R, et al. 2002. Mol. Biol. Cell. 13:317. 3. Mizumoto N, et al. 2004. J. Clin. Invest. 113:701. 4. Witte LD, et al. 2007. Nat. Med. 13:367.
Gene ID: 50489
UniProt: View information about CD207 on UniProt.org
Clone: 10E2
Regulatory Status: RUO
Other Names: CLEC4K
Isotype: Mouse IgG1, κ