Biolegend, 352309, PerCP/Cyanine5.5 anti-human CD138 (Syndecan-1) Antibody, 25tests

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human SDC1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns on in vitro generated plasma cells and some CD138+ cell lines.2 Additional reported applications (for the relevant formats of this clone) include: immunohistochemical staining in paraffin blocks of tissue sections1.
Application References(PubMed link indicates BioLegend citation): Osama MA. 2010. Int. J. Clin. Exp. Pathol. 3:280. (IHC) Itoua MR, et al. 2014. Biomed. Res. Int. 2014:536482. PubMed
Product Citations: SoRelle ED, et al. 2022. Front Immunol. 13:1001145. PubMed Kusner L, et al. 2014. PLoS One. 9:102231. PubMed Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed Garfall AL, et al. 2018. JCI Insight. 3. PubMed
RRID: AB_10895899 (BioLegend Cat. No. 352309) AB_10900979 (BioLegend Cat. No. 352310)
Structure: 100-200 kD type I integral transmembrane glycoprotein
Distribution: Plasma cells, pre-B cells, epithelial cells, endothelial cells
Function: Adhesion, control cell morphology, regulate cell growth
Ligand/Receptor: FGFb, collagen, fibronectin
Cell Type: B cells, Endothelial cells, Epithelial cells, Plasma cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Sanderson RD, et al. 1992. Cell. Regul. 1:27. 2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication.
Gene ID: 6382
UniProt: View information about CD138 on UniProt.org
Clone: DL-101
Regulatory Status: RUO
Workshop: V B045
Other Names: Syndecan-1, B-B4
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.