Biolegend, 352314, Alexa Fluor® 647 anti-human CD138 (Syndecan-1) Antibody, 100tests

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human SDC1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns on in vitro generated plasma cells and some CD138+ cell lines.2 Additional reported applications (for the relevant formats of this clone) include: immunohistochemical staining in paraffin blocks of tissue sections1.
Application References(PubMed link indicates BioLegend citation): Osama MA. 2010. Int. J. Clin. Exp. Pathol. 3:280. (IHC) Itoua MR, et al. 2014. Biomed. Res. Int. 2014:536482. PubMed
RRID: AB_2687335 (BioLegend Cat. No. 352313) AB_2687336 (BioLegend Cat. No. 352314)
Structure: 100-200 kD type I integral transmembrane glycoprotein
Distribution: Plasma cells, pre-B cells, epithelial cells, endothelial cells
Function: Adhesion, control cell morphology, regulate cell growth
Ligand/Receptor: FGFb, collagen, fibronectin
Cell Type: B cells, Endothelial cells, Epithelial cells, Plasma cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Sanderson RD, et al. 1992. Cell. Regul. 1:27. 2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication.
Gene ID: 6382
UniProt: View information about CD138 on UniProt.org
Clone: DL-101
Regulatory Status: RUO
Workshop: V B045
Other Names: Syndecan-1, B-B4
Isotype: Mouse IgG1, κ