Biolegend, 352505, APC anti-human GARP (LRRC32) Antibody, 25tests

Glycoprotein A Repetitions Predominant (GARP), also known as leucine rich repeat containing 32 (LRC32), is a 80 kD type I membrane glycoprotein with 20 leucine rich repeats in the extracellular portion of the protein. GARP was found on the surface of megakaryocytes, platelets, and activated Tregs (CD4+, CD25+, FoxP3+ cells) and serves as a receptor for latent TGF-β. Recent evidence suggests that GARP may play a role in controlling suppressor function of Tregs. A mutation in GARP has been reported in a large Samaritan kindred with Usher syndrome type 1, an autosomal recessive disease characterized by profound congenital sensorineural deafness, vestibular dysfunction, and progressive visual loss. In addition, it has been found that GARP mRNA is highly amplified in different tumors, which indicates that tumor cells may use GARP to express TGF-β or to capture TGF-β from their surroundings, resulting in local suppression of anti-tumor immune responses or the induction of Tregs.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: LRRC32-DNA vaccination
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Product Citations: Eschborn M, et al. 2015. J Allergy Clin Immunol. 136: 159-168. PubMed Chaumonnot K, et al. 2021. Cell Death Dis. 12:114. PubMed Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
RRID: AB_10896059 (BioLegend Cat. No. 352505) AB_10897808 (BioLegend Cat. No. 352506)
Structure: 80 kD transmembrane GARP glycoprotein with an extracellular region containing 20 leucine-rich repeats
Distribution: Activated Tregs, megakaryocytes, platelets
Ligand/Receptor: LAP (TGF-β1)
Cell Type: Megakaryocytes, Platelets, Tregs
Biology Area: Cell Biology, Immunology, Signal Transduction
Antigen References: 1. Ollendorff V, et al. 1994. Cell. Growth Differ. 5:213. 2. Stockis J, et al. 2009. Eur. J. Immunol. 39:3315. 3. Wang R, et al. 2009. P. Natl. Acad. Sci. USA 106:13439. 4. Tran DQ, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
Gene ID: 2615
UniProt: View information about GARP on UniProt.org
Clone: 7B11
Regulatory Status: RUO
Other Names: Garpin, Glycoprotein A repetitions predominant
Isotype: Mouse IgG2b, κ