Biolegend, 352908, Alexa Fluor® 488 anti-human EGFR Antibody, 100tests

Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein and member of the protein kinase superfamily that regulates cell growth and differentiation. EGFR binds EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor - all members of the EGF family. Ligand binding induces EGFR dimerization and autophosphorylation, initiating the MAPK, Akt, and JNK signaling pathways. EGFR is expressed by epithelial and endothelial cells and is frequently expressed by epithelial carcinomas.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Non-small cell lung cancer (NSCLC) cell line NCI-H322
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Yamaguchi M, et al. 2009. The 15th Annual Meeting Japan Society of Gene Therapy. p1056. Abstract 92.
Product Citations: Arshad N, et al. 2018. J Biol Chem. 293:9555. PubMed Pham TC, et al. 2021. J Extracell Vesicles. e12057:10. PubMed Kitamura Y, et al. 2021. Sci Adv. 7: . PubMed Mahameed M, et al. 2020. Nat Commun. 11:1304. PubMed Jayasinghe MK, et al. 2022. Theranostics. 12:3288. PubMed Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed Marrocco I, et al. 2021. EMBO Mol Med. 13:e13144. PubMed Mahameed M, et al. 2019. Cell Death Dis. 10:300. PubMed Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed Staudte S, et al. 2022. Cancers (Basel). 14:. PubMed Xuan Z, et al. 2020. Am J Cancer Res. 0.458333333. PubMed Liu H, et al. 2020. Cancer Discov. 1.268055556. PubMed Kahn S, et al. 2016. EMBO Mol Med. 8: 511 - 526. PubMed Leonard B, et al. 2018. Cancer Res. 78:4331. PubMed
RRID: AB_11124324 (BioLegend Cat. No. 352907) AB_11126165 (BioLegend Cat. No. 352908)
Structure: Member of the protein kinase superfamily; transmembrane glycoprotein; ligand binding induces dimerization and autophosphorylation
Distribution: Epithelial cells and endothelial cells; frequently found in epithelial carcinomas
Function: Controls cell growth and differentiation
Interaction: MAPK, Akt, JNK
Ligand/Receptor: Members of the epidermal growth factor (EGF) family such as EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor
Cell Type: Endothelial cells, Epithelial cells
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: CD Molecules, Growth Factors
Antigen References: 1. da Cunha Santos G, et al. 2011. Annu. Rev. Pathol. 6:49. 2. Gusterson BA and Hunter KD. 2009. Lancet Oncol. 10:522. 3. Mano M and Humblet Y. 2008. Nat. Clin. Pract. Oncol. 5:415. 4. Pao W and Chmielecki J. 2010. Nat. Rev. Cancer 10:760.
Gene ID: 1956
UniProt: View information about EGFR on UniProt.org
Clone: AY13
Regulatory Status: RUO
Other Names: Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, HER1
Isotype: Mouse IgG1, κ