Biolegend, 352919, Brilliant Violet 711™ anti-human EGFR Antibody, 25tests

Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein and member of the protein kinase superfamily that regulates cell growth and differentiation. EGFR binds EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor - all members of the EGF family. Ligand binding induces EGFR dimerization and autophosphorylation, initiating the MAPK, Akt, and JNK signaling pathways. EGFR is expressed by epithelial and endothelial cells and is frequently expressed by epithelial carcinomas.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Non-small cell lung cancer (NSCLC) cell line NCI-H322
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Yamaguchi M, et al. 2009. The 15th Annual Meeting Japan Society of Gene Therapy. p1056. Abstract 92.
Product Citations: Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
RRID: AB_2687122 (BioLegend Cat. No. 352919) AB_2687123 (BioLegend Cat. No. 352920)
Structure: Member of the protein kinase superfamily; transmembrane glycoprotein; ligand binding induces dimerization and autophosphorylation
Distribution: Epithelial cells and endothelial cells; frequently found in epithelial carcinomas
Function: Controls cell growth and differentiation
Interaction: MAPK, Akt, JNK
Ligand/Receptor: Members of the epidermal growth factor (EGF) family such as EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor
Cell Type: Endothelial cells, Epithelial cells
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: CD Molecules, Growth Factors
Antigen References: 1. da Cunha Santos G, et al. 2011. Annu. Rev. Pathol. 6:49. 2. Gusterson BA and Hunter KD. 2009. Lancet Oncol. 10:522. 3. Mano M and Humblet Y. 2008. Nat. Clin. Pract. Oncol. 5:415. 4. Pao W and Chmielecki J. 2010. Nat. Rev. Cancer 10:760.
Gene ID: 1956
UniProt: View information about EGFR on UniProt.org
Clone: AY13
Regulatory Status: RUO
Other Names: Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, HER1
Isotype: Mouse IgG1, κ