Biolegend, 353211, APC/Cyanine7 anti-human CD197 (CCR7) Antibody, 25tests

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CCR7-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Product Citations: Woodcock V, et al. 2023. BJUI Compass. 4:322. PubMed Toriyama M, et al. 2023. Front Mol Biosci. 10:1149828. PubMed Adamo S, et al. 2023. Cell Host Microbe. 31:928. PubMed Mo Y, et al. 2022. Front Immunol. 12:799896. PubMed Touizer E, et al. 2023. iScience. 26:105862. PubMed Meng Q, et al. 2016. PLoS One. 11:e0166954. PubMed Wu VH, et al. 2020. JCI Insight. 5:00. PubMed Kim C, et al. 2018. Cell Rep. 25:2148. PubMed Xiao C, et al. 2022. iScience. 25:103934. PubMed Tauriainen J, et al. 2017. Sci Rep. 7:40354. PubMed Hinks TSC, et al. 2019. Cell Rep. 28:3249. PubMed Demers K, et al. 2016. PLoS Pathog. 12: 1005805. PubMed Hong J, et al. 2014. J Immunol. 193:797. PubMed Kuri-Cervantes L, et al. 2020. STAR Protoc. 1:100154. PubMed Uchtenhagen H, et al. 2016. Nat Commun. 7:12614. PubMed Jankeel A, et al. 2020. J Virol. :94. PubMed Japp AS, et al. 2021. Cell. 184(3):827-839.e14. PubMed Alrubayyi A, et al. 2021. Nat Commun. 12:5839. PubMed Kuo HH, et al. 2018. Immunity. 48:1183. PubMed Kim C, et al. 2019. Cell Rep. 29:2202. PubMed Xu S, et al. 2021. Immunity. . PubMed Pattekar A, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 11(5):1267-1289. PubMed Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed Colomb F, et al. 2020. Cell Reports. 32(5):107991. PubMed Gao Y, et al. 2022. Immunity. 55:1732. PubMed Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed Poon MML, et al. 2021. Cell Rep. 37:110071. PubMed Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed Sekine T, et al. 2020. Cell. 183:158. PubMed NULL, et al. 2022. Cell. 185:916. PubMed Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed Meijgaarden K, et al. 2015. PLoS Pathog. 11:1004671. PubMed Crump NT, et al. 2021. Cell Reports. 35(6):109101. PubMed Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed Rusconi M, et al. 2017. Sci Rep. 5.745138889. PubMed Cortés–Rubio CN, et al. 2019. Clin Epigenetics. 11:134. PubMed
RRID: AB_10915272 (BioLegend Cat. No. 353211) AB_10916390 (BioLegend Cat. No. 353212)
Structure: Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution: T cells, B cells, NK, dendritic cells.
Function: The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor: CCL19 and CCL21.
Cell Type: B cells, Dendritic cells, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Yanagihara S, et al. 1998. J. Immunol. 161:3096. 2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610. 3. Reif K, et al. 2002. Nature 416:94. 4. Nakata B, et al. 2008. Oncology 74:69. 5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed 6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed 7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed
Gene ID: 1236
UniProt: View information about CD197 on UniProt.org
Clone: G043H7
Regulatory Status: RUO
Other Names: BLR2, CDw197, EBI1, CMKBR7
Isotype: Mouse IgG2a, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.