Biolegend, 353310, FITC anti-human Perforin Antibody, 100tests

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracelluar immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Li D, et al. 2023. Int J Oncol. 62:. PubMed Muralidhar A, et al. 2024. J Immunother Cancer. :12. PubMed Harari A, et al. 2021. Cancers (Basel). 13:. PubMed Chen M, et al. 2021. Cancers (Basel). 13:. PubMed Cohen KW, et al. 2021. Cell Reports Medicine. 2(7):100354. PubMed Garcia-Valtanen P, et al. 2022. Cell Rep Med. 3:100651. PubMed Graciotti M, et al. 2020. Vaccines (Basel). 8:00. PubMed
RRID: AB_2571966 (BioLegend Cat. No. 353309) AB_2571967 (BioLegend Cat. No. 353310)
Structure: 70 kD
Distribution: CTL, NK (cytoplasmic granules)
Function: Mediates targeted cell lysis.
Cell Type: NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361. 2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.
Gene ID: 5551
UniProt: View information about Perforin on UniProt.org
Clone: B-D48
Regulatory Status: RUO
Other Names: PRF1, P1, PFP, HPLH2
Isotype: Mouse IgG1