Biolegend, 353319, Alexa Fluor® 488 anti-human Perforin Antibody, 25tests

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Product Citations: Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed
RRID: AB_2783254 (BioLegend Cat. No. 353319) AB_2783255 (BioLegend Cat. No. 353320)
Structure: 70 kD
Distribution: CTL, NK (cytoplasmic granules)
Function: Mediates targeted cell lysis.
Cell Type: NK cells, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361. 2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.
Gene ID: 5551
UniProt: View information about Perforin on UniProt.org
Clone: B-D48
Regulatory Status: RUO
Other Names: PRF1, P1, PFP, HPLH2
Isotype: Mouse IgG1