Biolegend, 353402, Purified anti-human CD196 (CCR6) Antibody, 500μg

CCR6, also known as CD196, is a chemokine receptor that is expressed on immature dendritic cells, B lymphocytes, and memory T cells. CCR6 binds CCL20, although members of the β defensin family also bind CCR6 with a lower affinity. CCR6 positive cells, and its ligand CCL20, have been detected in numerous organs, especially the secondary lymphoid organ. CCL20 is selectively made by the follicle-associated epithelium (FAE) overlying Peyers Patches (PPs) and isolated lymphoid follicles (ILFs). CCL20 contributes to the recruitment of CCR6-expressing B cells to these structures. In humans, CCR6 can function to mediate arrest of T cells on dermal endothelial cells and is highly expressed on T cells resident in both normal and psoriatic skin. CCR6 and/or CCL20 have been implicated in the pathogenesis of rheumatoid arthritis and inflammatory bowel disease. Human T cells that are able to produce IL-17 express CCR6. It suggests that CCL20 and CCR6 have a role in inflammatory diseases by recruiting Th17 cells to target tissues.
500μg
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CCR6-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Spoerl S, et al. 2021. Eur J Immunol. 51:1436. PubMed McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed Sullivan KD, et al. 2021. Cell Reports. 36(7):109527. PubMed Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed Galbraith MD, et al. 2021. eLife. 10:00. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed Umeda M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
RRID: AB_10918626 (BioLegend Cat. No. 353401) AB_10918625 (BioLegend Cat. No. 353402)
Structure: Chemokine receptor, GPCR, seven transmembrane receptor
Distribution: Immature dendritic cells, B lymphocytes, memory T cells, Th17 cells
Function: Host defense, important for recruitment of B cells to secondary lymphoid organs, mediates arrest of T cells on dermal endothelial cells
Ligand/Receptor: CCL20 (MIP-3α)
Cell Type: B cells, Dendritic cells, T cells, Th17
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Zaballos A, et al. 1996. Biochem. Bioph. Res. Co. 227:846. 2. Yang D, et al. 1999. Science 286:525. 3. MacDonald KG, et al. 2007. Am. J. Pathol. 170:1229. 4. Homey B, et al. 2000. J. Immunol. 164:6621. 5. Hirota K, et al. 2007. J. Exp. Med. 204:2803. 6. Singh SP, et al. 2008. J. Immunol. 180:214.
Gene ID: 1235
UniProt: View information about CD196 on UniProt.org
Clone: G034E3
Regulatory Status: RUO
Other Names: BN-1, CKR6, DCR2, CKRL3, DRY-6, GPR29, CKR-L3, CMKBR6, GPRCY4, STRL22, GPR-CY4
Isotype: Mouse IgG2b, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.