Biolegend, 353602, Purified anti-human CD371 (CLEC12A) Antibody, 100μg

CD371 (CLEC12A), also known as DCAL-2, MICL or CLL-1, is a 30 kD type II transmembrane protein with extracellular C-type lectin domains, belonging to the C-type lectin family.  It is expressed on monocytes, granulocytes, NK cells, and basophils.  Its cytoplasmic ITIM motif modulates signaling cascades and is involved in phosphorylation of tyrosine residues in MAP kinases.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: 293T cells expressing CLEC12A-Flag
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Lahoud MH, et al. 2009. J. Immunol. 182:7587. (FC) Lahoud MH, et al. 2011. J. Immunol. 187:842. (FC) Neumann K, et al. 2014. Immunity. 40:389. PubMed
Product Citations: Neumann K, et al. 2014. Immunity. 40:389. PubMed Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed Gañán-Gómez I, et al. 2022. Nat Med. . PubMed
RRID: AB_10962440 (BioLegend Cat. No. 353602)
Structure: Type II C-type lectin family member, 265 aa, 30.7 kD
Distribution: Monocytes, granulocytes, NK cells, and basophils
Function: Negative regulator of granulocyte and monocyte function
Interaction: PTPN6 and PTPN11
Cell Type: Basophils, Dendritic cells, Granulocytes, Monocytes, NK cells
Biology Area: Immunology, Costimulatory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Lahoud MH, et al. 2009. J. Immunol. 182:7587. 2. Chen CH, et al. 2006. Blood 107:1459. 3. Marshall AS, et al. 2004. J. Biol. Chem. 279:14792.
Gene ID: 160364
UniProt: View information about CD371 on UniProt.org
Clone: 50C1
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: CLL1, MICL, CLL-1, DCAL-2, C-type lectin domain family 12 member A, CD371
Isotype: Mouse IgG2a, κ