Biolegend, 353610, PE/Cyanine7 anti-human CD371 (CLEC12A) Antibody, 100tests

CD371 (CLEC12A), also known as DCAL-2, MICL or CLL-1, is a 30 kD type II transmembrane protein with extracellular C-type lectin domains, belonging to the C-type lectin family.  It is expressed on monocytes, granulocytes, NK cells, and basophils.  Its cytoplasmic ITIM motif modulates signaling cascades and is involved in phosphorylation of tyrosine residues in MAP kinases.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: 293T cells expressing CLEC12A-Flag
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Lahoud MH, et al. 2009. J. Immunol. 182:7587. (FC) Lahoud MH, et al. 2011. J. Immunol. 187:842. (FC) Neumann K, et al. 2014. Immunity. 40:389. PubMed
RRID: AB_2563671 (BioLegend Cat. No. 353609) AB_2563672 (BioLegend Cat. No. 353610)
Structure: Type II C-type lectin family member, 265 aa, 30.7 kD
Distribution: Monocytes, granulocytes, NK cells, and basophils
Function: Negative regulator of granulocyte and monocyte function
Interaction: PTPN6 and PTPN11
Cell Type: Basophils, Dendritic cells, Granulocytes, Monocytes, NK cells
Biology Area: Immunology, Costimulatory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Lahoud MH, et al. 2009. J. Immunol. 182:7587. 2. Chen CH, et al. 2006. Blood 107:1459. 3. Marshall AS, et al. 2004. J. Biol. Chem. 279:14792.
Gene ID: 160364
UniProt: View information about CD371 on UniProt.org
Clone: 50C1
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: CLL1, MICL, CLL-1, DCAL-2, C-type lectin domain family 12 member A, CD371
Isotype: Mouse IgG2a, κ