Biolegend, 353730, Brilliant Violet 650™ anti-human CD183 (CXCR3) Antibody, 100tests

Human CXCR3, also known as GPR9, is a chemokine receptor that binds CXCL9, CXCL10, and CXCL11. It is a 38 kD seven-pass transmembrane receptor coupled to G-protein. CXCR3 is highly expressed by T cells (Th1), natural killer cells (NK cells), dendritic cells, mast cells, alveolar macrophages, eosinophils, and human airway epithelial cells. CXCR3 is important for effector lymphocyte recruitment into inflamed tissue in various inflammatory and autoimmune diseases, such as chronically inflamed liver, Crohn's disease, rheumatoid arthritis, multiple sclerosis, and inflammatory skin diseases.
100tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CXCR3 transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Product Citations: Rahman MA, et al. 2023. Nat Microbiol. 8:905. PubMed Kreutmair S, et al. 2021. Immunity. . PubMed Kenderes KJ, et al. 2018. Cell Rep. 24:824. PubMed Den Braanker H, et al. 2021. Front Immunol. 12:768113. PubMed Mudd PA, et al. 2022. Cell. 185:603. PubMed
RRID: AB_2562628 (BioLegend Cat. No. 353729) AB_2563870 (BioLegend Cat. No. 353730)
Structure: CXC-chemokine receptor, G protein-coupled receptor, seven-pass transmembrane receptor
Distribution: T cell subset, NK cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors, mast cells, alveolar macrophages, eosinophils, and airway epithelial cells
Function: Essential in T cell recruitment to sites of inflammation
Ligand/Receptor: CXCL9, CXCL10, and CXCL11
Cell Type: Dendritic cells, Eosinophils, Epithelial cells, Hematopoietic stem and progenitors, Macrophages, Mast cells, NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Loetscher M, et al. 1996. J. Exp. Med. 184:963. 2. Cole KE, et al. 1998. J. Exp. Med. 187:2009. 3. Aksoy MO, et al. 2006. Am. J. Physiol. Lung Cell Mol. Physiol. 290:L909. 4. Curbishley SM, et al. 2005. Am. J. Pathol. 167:887. 5. Turner JE, et al. 2007. Mini. Rev. Med. Chem. 7:1089. 6. Wenzel J, et al. 2008. J. Invest. Dermatol. 128:67.
Gene ID: 2833
UniProt: View information about CD183 on UniProt.org
Clone: G025H7
Regulatory Status: RUO
Other Names: CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2
Isotype: Mouse IgG1, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.