Biolegend, 353903, PE anti-human CD365 (Tim-1) Antibody, 25tests

CD365 (Tim-1) is a transmembrane glycoprotein expressed on activated CD4 + lymphocytes especially on Th2 cells. It has been implicated to play a critical role in the development of atopic disease and other Th2-biased immune responses. Tim-1 is a hepatitis A virus receptor in humans. Tim-4 is the endogenous ligand of Tim-1. The interaction of Tim-1 and Tim-4 is involved in the costimulation of T cell proliferation. Tim-1 has been reported to be an endogenous ligand for LMIR5 (Leukocyte mono-immunoglobulin (Ig)-like receptor 5), and the Tim-1-LMIR5 interaction plays a physiological role in immune regulation by myeloid cells.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human TIM-1-IgV Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported application (for the relevant formats) include: blocking function1.
Application References(PubMed link indicates BioLegend citation): Freeman GJ, et al. 2007. Immunity. 27:927. (Block)
Product Citations: Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Aravena O, et al. 2017. Arthritis Res Ther. 19:8. PubMed Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed Smith CM, et al. 2021. Biochem J. 478:3331. PubMed
RRID: AB_11125165 (BioLegend Cat. No. 353903) AB_11125175 (BioLegend Cat. No. 353904)
Structure: Type 1 transmembrane glycoprotein.
Distribution: Activated CD4+ lymphocytes, especially on Th2 cells.
Function: TIM-1 ligation induces T cell proliferation, promotes cytokine production, and aids in the regulation of Th2 immunity.
Ligand/Receptor: TIM-4
Cell Type: Dendritic cells, T cells, Th2
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Freeman GJ, et al. 2007. Immunity. 27:927. 2. Meyers JH, et al. 2005. Nat. Immunol. 6:455. 3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454. 4. Mariat C, et al. 2005. Philos. Trans. R. Soc. Lond. B. 360:1681. 5. Yamanishi Y, et al. 2010. J. Exp. Med. 207:1501.
Gene ID: 26762
UniProt: View information about CD365 on UniProt.org
Clone: 1D12
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T cell immunoglobulin mucin 1, TIMD-1, KIM-1 (kidney injury molecule-1), HAVCR-1, CD365
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.