Biolegend, 354106, APC anti-human CD89 Antibody, 100tests

CD89, also known as FcαR, is a 55-100 kD glycosylated protein.  It belongs to the immunoglobulin gene family.  It is expressed on granulocytes, monocytes, and macrophages but is absent on T cells.  It can interact with IgA aggregates and plays an important role in IgA mediated immune responses.
100tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Ag8.653 myeloma cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Monteiro RC, et al. 1992. J. Immunol. 148:1764. Shen L. 1992. J. Leukoc. Biol. 51:373. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Rogers KA, et al. 2004. Immunology 113:178.
Product Citations: Fazekas–Singer J, et al. 2018. J Allergy Clin Immunol. 142:973. PubMed Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed Lee WS, et al. 2021. Cell Rep Med. 2:100296. PubMed Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID: AB_2565256 (BioLegend Cat. No. 354105) AB_2565257 (BioLegend Cat. No. 354106)
Structure: Member of the immunoglobulin gene family and is a transmembrane glycoprotein, 55-100 kD
Distribution: Granulocytes, neutrophils, eosinophils, monocytes, macrophages
Function: Interacts with IgA opsonized targets and induces immunological processes such as phagocytosis, ADCC, and release of inflammatory mediators
Ligand/Receptor: IgA (monomeric, dimeric, polymeric and secretory IgA)
Cell Type: Eosinophils, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area: Immunology
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Patry C, et al. 1996. J. Immunol. 156:4442. 2. de Wit, et al. 1995. J. Immunol. 155:1203. 3. Honorio-França AC, et al. 2001. J. Leukoc. Biol. 69:289.
Gene ID: 2204
UniProt: View information about CD89 on UniProt.org
Clone: A59
Regulatory Status: RUO
Workshop: V MR30
Other Names: FcαR
Isotype: Mouse IgG1, κ