Biolegend, 354210, PerCP/Cyanine5.5 anti-human CD303 (BDCA-2) Antibody, 100tests

CD303, also known as BDCA-2 and CLEC4C, is a 38 kD type II transmembrane glycoprotein. It is a member of the C-type lectin superfamily. CD303 is expressed by plasmacytoid dendritic cells (pDCs) and is involved in cell adhesion, signaling, and antigen capture and processing. Crosslinking of CD303 inhibits the production of IFN-α/β and TLR-9 induced pDCs maturation.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Freeman A, et al. 2014. PLoS One. 9:110928. PubMed Johnson P, et al. 2015. Clin Cnacer Res. 21:1321. PubMed
Product Citations: Robinson GA, et al. 2021. EBioMedicine. 103243:. PubMed Grenga I, et al. 2016. Clin Transl Immunology. 0.265972222. PubMed Moen IN, et al. 2021. Cell Death Discov. 7:36. PubMed Heger L, et al. 2018. Front Immunol. 9:744. PubMed Autenrieth S, et al. 2015. Clin Transl Immunology. 4:50. PubMed Vaeth M, et al. 2015. J Immunol. 195: 1202-1217. PubMed Breton G, et al. 2015. J Exp Med. 212:401. PubMed Sponaas A, et al. 2015. PLoS One. 10: e0139867. PubMed
RRID: AB_11219596 (BioLegend Cat. No. 354209) AB_11219604 (BioLegend Cat. No. 354210)
Structure: Type II transmembrane protein, 38 kD, member of the C-type lectin superfamily
Distribution: Plasmacytoid dendritic cells
Function: Antigen capture, inhibition of IFN α/β production
Cell Type: Dendritic cells
Biology Area: Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Jähn PS, et al. 2010. Cell Immunol. 265:15. 2. Graham LM and Brown GD. 2009. Cytokine 48:148. 3. Röck J, et al. 2007. Eur. J. Immunol. 37:3564. 4. Dzionek A, et al. 2001. J. Exp. Med. 194:1823.
Gene ID: 170482
UniProt: View information about CD303 on UniProt.org
Clone: 201A
Regulatory Status: RUO
Other Names: CLEC4C, CLECSF11, CLECSF7, DLEC, HECL
Isotype: Mouse IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.